Strain Name:
C.129P2(Cg)-Cftrtm1Unc/Mmnc
Stock Number:
029585-UNC
Citation ID:
RRID:MMRRC_029585-UNC
Other Names:
Cystic fibrosis transmembrane regulator

Strain Information

Cftrtm1Unc
Name: cystic fibrosis transmembrane conductance regulator; targeted mutation 1, University of North Carolina
Synonyms: Cftr-, UNC, mCFTR-, CftrUNC, CFTR S489X-, S489X, p.S489*, cftrm1UNC, Cftr-.ko
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
Cftr
Name: cystic fibrosis transmembrane conductance regulator
Synonyms: Abcc7
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knockout
NCBI: 12638
HGNC: HGNC:1884
Homologene: 55465
Genetic Alterations
A neomycin cassette was inserted into exon 10. This resulted in a truncation mutation. Low levels of the truncated CFTR mRNA can be detected.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    E14TG2a derived from 129P2/OlaHsd
    Phenotype
    Homozygous: Homozygous mice display many features common to human cystic fibrosis patients, including failure to thrive, meconium ileus, alteration of mucous and serous glands, and obtstruction of glandlike structures with inspissated eosinophilic material. Death resulting from intestinal obstruction usually occurs before 40 days of age, if mice are not supplemented with colyte.

    Heterozygous: Heterozygous mice are normal and fertile.
    Mammalian Phenotype Terms
    Allelic Composition: Cftrtm1Unc/Cftrtm1Unc (Genetic Background: B6.129P2-Cftrtm1Unc )

    Allelic Composition: Cftrtm1Unc/Cftrtm1Unc (Genetic Background: B6.129P2-Cftrtm1Unc/J )

    Allelic Composition: Cftrtm1Unc/Cftrtm1Unc (Genetic Background: involves: 129P2/OlaHsd )

    Allelic Composition: Cftrtm1Unc/Cftrtm1Unc (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * FVB/N )

    Allelic Composition: Cftrtm1Unc/Cftrtm1Unc (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

    Allelic Composition: Cftrtm1Unc/Cftr+ (Genetic Background: involves: 129P2/OlaHsd )

    Allelic Composition: Cftrtm1Unc/Cftr+ (Genetic Background: involves: BALB/cJ * C57BL/6J )

    MeSH Terms
    • Amino Acid Sequence
    • Animals
    • Base Sequence
    • Blotting, Southern
    • Cells, Cultured
    • Codon
    • Cystic Fibrosis/genetics
    • Cystic Fibrosis Transmembrane Conductance Regulator
    • DNA/genetics
    • Disease Models, Animal
    • Drug Resistance, Microbial/genetics
    • Embryo, Mammalian
    • Exons
    • Genetic Techniques
    • Kanamycin Kinase
    • Membrane Proteins/genetics
    • Mice
    • Molecular Sequence Data
    • Neomycin/pharmacology
    • Oligodeoxyribonucleotides
    • Phosphotransferases/genetics
    • Plasmids
    • Polymerase Chain Reaction/methods
    • Promoter Regions, Genetic
    • Restriction Mapping
    • Simplexvirus/genetics
    • Stem Cells/physiology
    • Thymidine Kinase/genetics
    • Cystic Fibrosis/pathology
    • Cystic Fibrosis/physiopathology
    • Digestive System/metabolism
    • Digestive System/pathology
    • Exocrine Glands/pathology
    • Gallbladder/pathology
    • Genitalia, Male/pathology
    • Genotype
    • Growth
    • Intestinal Obstruction/etiology
    • Intestinal Obstruction/pathology
    • Liver/pathology
    • Male
    • Meconium/metabolism
    • Mice, Inbred BALB C
    • Mice, Inbred C57BL
    • Mucus/metabolism
    • Mutagenesis
    • Pancreas/pathology
    • RNA, Messenger/metabolism
    • Salivary Glands/pathology
    • Chloride Channels/genetics
    • Colonic Diseases/pathology
    • Disease Susceptibility
    • Female
    • Fertility
    • Gene Targeting
    • Humans
    • Lung Diseases/microbiology
    • Lung Diseases/pathology
    • Mice, Transgenic
    • Pancreatic Diseases/pathology
    • Pancreatic Ducts/pathology
    • Sequence Deletion/genetics
    • Specific Pathogen-Free Organisms
    • Staphylococcal Infections/pathology
    • Survival Rate
    Strain Development
    Twelve consecutive crosses to purchased BALB/cJ mice.
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Immunology and Inflammation
    • Models for Human Disease
    Donor
    Beverly Koller, Ph.D., University of North Carolina at Chapel Hill
    Primary Reference
    • Koller BH, Kim HS, Latour AM, Brigman K, Boucher RC Jr, Scambler P, Wainwright B, Smithies O. Toward an animal model of cystic fibrosis: targeted interruption of exon 10 of the cystic fibrosis transmembrane regulator gene in embryonic stem cells. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10730-4. (Medline PMID: 1720548)
    • Snouwaert JN, Brigman KK, Latour AM, Malouf NN, Boucher RC, Smithies O, Koller BH. An animal model for cystic fibrosis made by gene targeting. Science. 1992 Aug 21;257(5073):1083-8. (Medline PMID: 1380723)
    • Snouwaert JN, Brigman KK, Latour AM, Iraj E, Schwab U, Gilmour MI, Koller BH. A murine model of cystic fibrosis. Am J Respir Crit Care Med. 1995 Mar;151(3 Pt 2):S59-64. (Medline PMID: 7533607)

    Colony and Husbandry Information

    -/- animals must be given water supplemented with colyte (PEG-3350) in order to survive.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    029585-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.