Strain Name:
B6;129X1-Ptk2tm1Lfr/Mmucd
Stock Number:
009967-UCD
Citation ID:
RRID:MMRRC_009967-UCD
Other Names:
fak-flox, B6;129X1-Ptk2tm1Lfr/Mmcd

Gene Information

Allele Symbol: Ptk2tm1Lfr (Mus musculus (mouse))
Name: PTK2 protein tyrosine kinase 2; targeted mutation 1, Louis F Reichardt
Synonyms: FAX, fak-flox
Chromosome: 15
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:2684666
Gene Symbol: Ptk2 (Mus musculus (mouse))
Name: PTK2 protein tyrosine kinase 2
Synonyms: FRNK, FAK, Fadk
Chromosome: 15
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:95481
Genetic Alterations
Floxed allele of focal adhesion kinase
Genotype Determination
ES Cell Line
JM-1 derived from 129X1/SvJ
Phenotype
Homozygous and Heterozygous mice do not have a phenotype unless crossed with cre.

MeSH Terms
  • Animals
  • Astrocytes/metabolism
  • Astrocytes/pathology
  • Bacterial Proteins/metabolism
  • Basement Membrane/metabolism
  • Basement Membrane/pathology
  • Blotting, Western
  • Calbindin 2
  • Calbindins
  • Carrier Proteins/metabolism
  • Cell Adhesion Molecules, Neuronal/metabolism
  • Cells, Cultured
  • Cerebral Cortex/abnormalities
  • Cerebral Cortex/metabolism
  • Cerebral Cortex/pathology
  • Cerebral Cortex/ultrastructure
  • Cytoskeletal Proteins/metabolism
  • DNA-Binding Proteins/metabolism
  • Disease Models, Animal
  • Dura Mater
  • Dystroglycans
  • Embryo, Mammalian
  • Extracellular Matrix Proteins/metabolism
  • Fibroblasts/metabolism
  • Fibroblasts/virology
  • Focal Adhesion Kinase 1
  • Focal Adhesion Protein-Tyrosine Kinases
  • Glial Fibrillary Acidic Protein/metabolism
  • Heterozygote
  • Homeodomain Proteins/metabolism
  • Immunohistochemistry
  • Infection
  • Lamins/metabolism
  • Membrane Glycoproteins/metabolism
  • Mice
  • Mice, Knockout
  • Microscopy, Electron
  • Microtubule-Associated Proteins/metabolism
  • Muscular Dystrophies/congenital
  • Muscular Dystrophies/genetics
  • Muscular Dystrophies/metabolism
  • Muscular Dystrophies/pathology
  • Mutation
  • Nerve Tissue Proteins
  • Neurons/metabolism
  • Neurons/pathology
  • Otx Transcription Factors
  • Phosphopyruvate Hydratase/metabolism
  • Phosphotyrosine/metabolism
  • Precipitin Tests
  • Protein-Tyrosine Kinases/deficiency
  • Protein-Tyrosine Kinases/genetics
  • Protein-Tyrosine Kinases/metabolism
  • S100 Calcium Binding Protein G/metabolism
  • Serine Endopeptidases
  • Silver Staining
  • Staining and Labeling
  • Transcription Factors/metabolism
  • src-Family Kinases/metabolism
Strain Development

Specific deletion of FAK in the anlage of the dorsal telendin- cephalon was accomplished by mating fak-flox mice to mice expressing Cre recombinase under the control of the endogenous emx1 promoter (Gorski et al., 2002). Received mice were tested using microsattelite analysis, and determined to be approximately N2 backcross to C57BL/6. The MMRRC will cryorecover using C57BL/6NHsd.

Suggested Control Mice
Wild-type littermates
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Hematology
  • Immunology and Inflammation
  • Models for Human Disease
  • Neurobiology
  • Sensorineural
Donor
Louis F. Reichardt, Ph.D., University of California, San Francisco
Primary Reference
Beggs HE, Schahin-Reed D, Zang K, Goebbels S, Nave KA, Gorski J, Jones KR, Sretavan D, Reichardt LF. FAK deficiency in cells contributing to the basal lamina results in cortical abnormalities resembling congenital muscular dystrophies. Neuron. 2003 Oct 30;40(3):501-14. (Medline PMID: 14642275)

Colony and Husbandry Informationon

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
MMRRC Breeding System
The MMRRC has always maintained this strain in a cryopreserved format. Information in the "Breeding" and "Reproductive statistics" sections has been provided by the Donating Investigator
Breeding Scheme(s)
  • C57BL/6 female x Heterozygous male
Generation
N3 As of: 1/2005 (verified by micro-satellite testing)
Overall Breeding Performance
Good
Average litter size
8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
009967-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
009967-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2
009967-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.