Strain Name:
B6.129-Dysftm1Kcam/Mmmh
Stock Number:
010317-MU
Citation ID:
RRID:MMRRC_010317-MU

Gene Information

Allele Symbol: Dysftm1Kcam (Mus musculus (mouse))
Name: dysferlin; targeted mutation 1, Kevin P Campbell
Chromosome: 6
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:2661116
Gene Symbol: Dysf (Mus musculus (mouse))
Name: dysferlin
Synonyms: 2310004N10Rik
Chromosome: 6
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:1349385
Genetic Alterations
The targeting vector was constructed to delete the last three coding exons including the exons coding for the transmembrane domain of the dysferlin gene.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Dysferlin-null mice are viable and have a normal growth rate, but develop a progressive muscular dystrophy.

Heterozyous: Heterzygotes do not show any known morphological abnormalities.
Mammalian Phenotype Terms
Allelic Composition: Dysftm1Kcam/Dysftm1Kcam (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 )

MeSH Terms
  • Animals
  • Calcium/metabolism
  • Cell Membrane/pathology
  • Dysferlin
  • Dystrophin/metabolism
  • Gene Deletion
  • Membrane Fusion
  • Membrane Proteins
  • Mice
  • Mice, Knockout
  • Muscle Fibers, Skeletal/metabolism
  • Muscle Fibers, Skeletal/pathology
  • Muscle Proteins/deficiency
  • Muscle Proteins/genetics
  • Muscle Proteins/metabolism
  • Muscle, Skeletal/metabolism
  • Muscle, Skeletal/pathology
  • Muscular Dystrophies/genetics
  • Muscular Dystrophies/metabolism
  • Muscular Dystrophies/pathology
  • Muscular Dystrophies/physiopathology
  • Regeneration
  • Stress, Mechanical
Suggested Control Mice
  • Wildtype littermates
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
Donor
Kevin P. Campbell, Ph.D., University of Iowa
Primary Reference
Bansal D, Miyake K, Vogel SS, Groh S, Chen CC, Williamson R, McNeil PL, Campbell KP. Defective membrane repair in dysferlin-deficient muscular dystrophy. Nature. 2003 May 8;423(6936):168-72. (Medline PMID: 12736685)

Colony and Husbandry Informationon

Sometimes teeth need to be clipped due to malocclusion of teeth. May need to gruel pups up to 2 weeks after weaning or as needed.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
010317-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity2
010317-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.