Strain Name:
Stock Number:
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Gene Information

Allele Symbol: S1pr1tm1Rlp [MGI:2158346] (Mus musculus (mouse))
Name: sphingosine-1-phosphate receptor 1; targeted mutation 1, Richard L Proia
Synonyms: S1pr1-, Slp1-LacZ, S1P1Ko, S1P1-, Edg1LacZ
Chromosome: 3
Alteration at locus: Knockout
Gene Symbol: S1pr1 [MGI:1096355] (Mus musculus (mouse))
Name: sphingosine-1-phosphate receptor 1
Synonyms: S1P, Edg1, S1P1
Chromosome: 3
Alteration at locus: Knockout
Genetic Alterations
LacZ-neo was inserted into exon 2 of the target gene.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    TC1/TC-1 derived from 129S6/SvEvTac
    Embryonic lethal at E12.5-13.5; massive bleeding; defect in vascular maturation.
    Mammalian Phenotype Terms
    Allelic Composition: S1pr1tm1Rlp/S1pr1tm1Rlp (Genetic Background: involves: 129S6/SvEvTac )

    Allelic Composition: S1pr1tm1Rlp/S1pr1tm1Rlp (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 )

    MeSH Terms
    • Animals
    • Blood Vessels/embryology
    • Cardiovascular System/embryology
    • Cell Movement
    • Fibroblasts/cytology
    • Fibroblasts/drug effects
    • GTP-Binding Proteins/metabolism
    • Heart/embryology
    • Homozygote
    • Immediate-Early Proteins/genetics
    • Lysophospholipids
    • Mice
    • Mice, Knockout
    • Muscle, Smooth, Vascular/embryology
    • Phenotype
    • Receptors, Cell Surface
    • Receptors, G-Protein-Coupled
    • Receptors, Lysophospholipid
    • Signal Transduction
    • Sphingosine/analogs & derivatives
    • Sphingosine/metabolism
    • Sphingosine/pharmacology
    Strain Development
    129S6-derived TC-1 ES cells were injected into C57BL/6 blastocysts. Resulting chimeric mice were backcrossed to C57BL/6. Heterozygous siblings then were mated.
    Suggested Control Mice
    • Wildtype littermates
    • Blood vessel development
    Dr. Richard Proia, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health
    Primary Reference

    Liu, Y., Wada, R. Yamashita, T., Mi, Y., Deng, C.X., Hobson, J.P., Rosenfeldt, H.M., Nava, V.E., Chae, S.S., Lee, M.J., Liu, C.H., Hla, T., Spiegel, S., and Proia, R.L. (2000) Edg-1, the G protein-coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation. J. Clin. Invest. 106(8):951-961. (Medline PMID: 11032855)

    Colony and Husbandry Information

    Maintained in Techniplast ventilated caging, autoclaved cages and water, irradiated feed (PicoLab). Cages are changed in hepa filtered hood. Technicians wear Tyvek suits, head covers, masks, shoe covers, gloves. Gloved hands are dipped in disinfectant when handling cages, mice. Access to breeding area limited to MMRRC personnel.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .
    Coat Color
    Black, brown
    MMRRC Breeding System
    Intra-strain mate heterozygotes or back-cross to C57BL/6
    Breeding Scheme(s)
    • Heterozygous Female x Heterozygous Male
    • Heterozygote x C57BL/6
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: No No Homozygotes die in utero from d.9.5-13.5 due to hemorrhage. Disruption of gene causes impairment of recruitment of smooth muscle cell to vessel walls.
    Homozygotes are fertile: No No
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Average litter size
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    000035-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to for more information.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.