Allele Symbol: S1pr1tm1Rlp
Name: targeted mutation 1, Richard L Proia
Alteration at locus: Knockout
Gene Symbol: S1pr1
Name: sphingosine-1-phosphate receptor 1
Alteration at locus: Knockout
ES Cell Line: TC1/TC-1
Embryonic lethal at E12.5-13.5; massive bleeding; defect in vascular maturation.Mammalian Phenotype Terms:
Strain of Origin: B6;129S6
Strain genetic background: B6;129S6
Strain Development: 129S6-derived TC-1 ES cells were injected into C57BL/6 blastocysts. Resulting chimeric mice were backcrossed to C57BL/6. Heterozygous siblings then were mated.
Suggested Control Mice:
Donor: Dr. Richard Proia, National Institute of Diabetes and Digestive and Kidney Diseases, NIH
Liu, Y., Wada, R. Yamashita, T., Mi, Y., Deng, C.X., Hobson, J.P., Rosenfeldt, H.M., Nava, V.E., Chae, S.S., Lee, M.J., Liu, C.H., Hla, T., Spiegel, S., and Proia, R.L. (2000) Edg-1, the G protein-coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation. J. Clin. Invest. 106(8):951-961. (Medline: 11032855)
Maintained in Techniplast ventilated caging, autoclaved cages and water, irradiated feed (PicoLab). Cages are changed in hepa filtered hood. Technicians wear Tyvek suits, head covers, masks, shoe covers, gloves. Gloved hands are dipped in disinfectant when handling cages, mice. Access to breeding area limited to MMRRC personnel.
Coat color: Black, brown
MMRRC Breeding System: Intra-strain mate heterozygotes or back-cross to C57BL/6
Overall Breeding Performance:
Breeding Comments: Intra-strain mate heterozygotes, heterozygote x wild type, or backcross to C57BL/6J
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||No||No||Homozygotes die in utero from d.9.5-13.5 due to hemorrhage. Disruption of gene causes impairment of recruitment of smooth muscle cell to vessel walls.|
|Homozygotes are fertile:||No||No|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Unknown||Unknown|
Average litter size: 5-6
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.