Strain Name:
B6;SJL-Nos2+ Tg(APPSWE)2576Kha Tg(PSEN1)1Zhe/Mmnc
Stock Number:
011170-UNC
Citation ID:
RRID:MMRRC_011170-UNC

Gene Information

Allele Symbol: Nos2+ (Mus musculus (mouse))
Name: nitric oxide synthase 2, inducible; wild type
Chromosome: 11
Alteration at locus: Non-mutant
Monarch Initiative (Disease and Phenotype Associations): MGI:2152807
Gene Symbol: Nos2 (Mus musculus (mouse))
Name: nitric oxide synthase 2, inducible
Synonyms: iNOS, Nos2a, NOS-II, Nos-2
Chromosome: 11
Alteration at locus: Non-mutant
Monarch Initiative (Disease and Phenotype Associations): MGI:97361
Transgene: App (Mus musculus (mouse))
Name: amyloid beta (A4) precursor protein
Synonyms: Cvap, Adap, E030013M08Rik, Abeta, appican, protease nexin II, betaAPP
Chromosome: 16
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations): MGI:88059
Transgene: PSEN1 (Homo sapiens (human))
Name: presenilin 1 (Alzheimer disease 3)
Chromosome: 14
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations):
Transgene Symbol: Tg(APPSWE)2576Kha (Mus musculus (mouse))
Name: transgene insertion 2576, Karen Hsiao Ashe; transgene insertion 2576, Karen Hsiao Ashe
Synonyms: APPswe Tg2576, Tg(APPSWE)2576HKahs, K670N/M671L, APP695swe, APPSw, Tg2576, Tg(HuAPP695.K670-M671L)2576, TgN(APPSWE)2576, Tg(APPSWE)2576Kahs, APPK670N,M671L, hAPP
Chromosome: unknown
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations): MGI:2385631
Genetic Alterations
For iNOS knockout, donor deleted 585bp of promoter plus exons 1-4 while inserting neo cassette (Cell 81: 641, 1995). The transgenes were introduced by others into separate strains and brought together with the ko as described above. "Recessive" designation below refers to disrupted iNOS alleles.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    N/A
    Phenotype
    Homozygous: Alzheimer's disease-like brain pathology and premature mortality
    Hemizygous: not characterized
    MeSH Terms
    • Alzheimer Disease/enzymology
    • Alzheimer Disease/genetics
    • Alzheimer Disease/mortality
    • Alzheimer Disease/pathology
    • Animals
    • Brain/enzymology
    • Brain/pathology
    • Crosses, Genetic
    • Disease Models, Animal
    • Gene Deletion
    • Humans
    • Inbreeding
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Mice, Transgenic
    • Nitric Oxide Synthase Type II/deficiency
    • Nitric Oxide Synthase Type II/genetics
    Strain Development
    First, donor backcrossed the original iNOS-/- C57BL/6129 mice (30) to C57BL/6 for 6 generations. Descendants of brother-sister matings of the latter mice were crossed with the SJL strain and their progeny interbred to derive iNOS-/- C57BL/6SJL mice. This step was based on evidence that that modifier genes from the SJL background were critical to avoid premature mortality in C57BL/6 mice bearing a mutant (K670N, M671L) human amyloid precursor protein (APP) transgene (31). Donor bred the iNOS-/- C57BL/6SJL mice with a strain called Tg2576 in which a hamster prion promoter drives the K670N, M671L APP transgene in the C57Bl/6SJL background (32), and also with transgenics in which the Thy1 promoter drives human presenilin-1 (hPS1) with the A246E mutation in the C57Bl/6SJL background (33). Donor interbred the progeny to establish three C57BL/6SJL sublines from littermates: (i) wild type mice (iNOS+/+ hAPP0/0 hPS10/0); (ii) mice with wild type iNOS alleles and the APP and PS1 transgenes, each of which was inherited from only one parent so as to avoid overdose (iNOS+/+ hAPP+/0 hPS1+/0); and (iii) mice with disrupted iNOS alleles and the APP and PS1 transgenes (iNOS-/- hAPP+/0 hPS1+/0).
    Suggested Control Mice
    • Wildtype littermates
    • Immunology and Inflammation
    • Models for Human Disease
    Donor
    Carl Nathan, M.D., Weill Cornell Medical College
    Primary Reference
    Nathan C, Calingasan N, Nezezon J, Ding A, Lucia MS, La Perle K, Fuortes M, Lin M, Ehrt S, Kwon NS, Chen J, Vodovotz Y, Kipiani K, Beal MF. Protection from Alzheimer's-like disease in the mouse by genetic ablation of inducible nitric oxide synthase. J Exp Med. 2005 Nov 7;202(9):1163-9. Epub 2005 Oct 31. (Medline PMID: 16260491)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

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