For the nitric oxide synthase (Nos2) knockout, 585 bp of the promoter and exons 1-4 were deleted while inserting a neo cassette (PMID:7538909). Two additional transgenes were brought together with the KO, where (A) the gold hamster prion protein (Prnp) promoter drives the expression of human amyloid beta (A4) precursor protein (APP, NCBI Gene:351) with the Swedish mutation (K670N/M671L), and (B) the human thymus cell antigen 1, theta (THY1, NCBI Gene:7070) promoter drives the expression of human presenilin 1 (PSEN1, NCBI Gene:5663) with the A246E mutation. In this particular strain, the KO allele is not present through coordinated interbreeding (i.e., wild-type).
Wild-type littermates - MMRRC:11169
Nathan C, Calingasan N, Nezezon J, Ding A, Lucia MS, La Perle K, Fuortes M, Lin M, Ehrt S, Kwon NS, Chen J, Vodovotz Y, Kipiani K, Beal MF. Protection from Alzheimer's-like disease in the mouse by genetic ablation of inducible nitric oxide synthase. J Exp Med. 2005 Nov 7;202(9):1163-9. Epub 2005 Oct 31. (Medline PMID: 16260491)
Hsiao K, Chapman P, Nilsen S, Eckman C, Harigaya Y, Younkin S, Yang F, Cole G. Correlative memory deficits, Abeta elevation, and amyloid plaques in transgenic mice. Science. 1996 Oct 4;274(5284):99-102. (Medline PMID: 8810256)
MacMicking JD, Nathan C, Hom G, Chartrain N, Fletcher DS, Trumbauer M, Stevens K, Xie QW, Sokol K, Hutchinson N, et al. Altered responses to bacterial infection and endotoxic shock in mice lacking inducible nitric oxide synthase. Cell. 1995 May 19;81(4):641-50. Erratum in: Cell 1995 Jun 30;81(7):following 1170. (Medline PMID: 7538909)
Rodrigo J, Fernández-Vizarra P, Castro-Blanco S, Bentura ML, Nieto M, Gómez-Isla T, Martínez-Murillo R, MartInez A, Serrano J, Fernández AP. Nitric oxide in the cerebral cortex of amyloid-precursor protein (SW) Tg2576 transgenic mice. Neuroscience. 2004;128(1):73-89. (Medline PMID: 15450355)
Qian S, Jiang P, Guan XM, Singh G, Trumbauer ME, Yu H, Chen HY, Van de Ploeg LH, Zheng H. Mutant human presenilin 1 protects presenilin 1 null mouse against embryonic lethality and elevates Abeta1-42/43 expression. Neuron. 1998 Mar;20(3):611-7. (Medline PMID: 9539133)
Mullan M, Crawford F, Axelman K, Houlden H, Lilius L, Winblad B, Lannfelt L. A pathogenic mutation for probable Alzheimer's disease in the APP gene at the N-terminus of beta-amyloid. Nat Genet. 1992 Aug;1(5):345-7. (Medline PMID: 1302033)Hsiao KK, Borchelt DR, Olson K, Johannsdottir R, Kitt C, Yunis W, Xu S, Eckman C, Younkin S, Price D, et al. Age-related CNS disorder and early death in transgenic FVB/N mice overexpressing Alzheimer amyloid precursor proteins. Neuron. 1995 Nov;15(5):1203-18. (Medline PMID: 7576662))
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||>12 months||>12 months|
When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to firstname.lastname@example.org for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.