Strain Detail Sheet

Strain Name:
STOCK Nos2+/Mmnc
Stock Number:
011169-UNC
Citation ID:
RRID:MMRRC_011169-UNC
Other Names:
STOCK/Mmnc

Strain Information

Gene Details

Nos2+
Name: nitric oxide synthase 2, inducible; wild type
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Non-mutant
Nos2
Name: nitric oxide synthase 2, inducible
Synonyms: iNOS, Nos-2, Nos2a, NOS-II
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Non-mutant
NCBI: 18126
HGNC: HGNC:7873
Homologene: 55473
Genome Browser [Click image to go to Jbrowse]



Additional Resources
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ClinGen
Genetic Alterations

Wild-type control for MMRRC:11170 and 11171

Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.

    • Strain Description

    Phenotype
    Normal
    MeSH Terms
    • Aging
    • Alzheimer Disease/metabolism
    • Alzheimer Disease/pathology
    • Amyloid beta-Peptides/analysis
    • Amyloid beta-Protein Precursor/analysis
    • Amyloid beta-Protein Precursor/genetics
    • Animals
    • Brain/pathology
    • Brain Chemistry
    • Learning Disabilities/metabolism
    • Learning Disabilities/pathology
    • Maze Learning
    • Memory Disorders/metabolism
    • Memory Disorders/pathology
    • Mice
    • Mice, Transgenic
    • Peptide Fragments/analysis
    • Psychomotor Performance
    • Amino Acid Oxidoreductases/deficiency
    • Amino Acid Oxidoreductases/genetics
    • Bacterial Infections/metabolism
    • Base Sequence
    • Mice, Mutant Strains
    • Molecular Sequence Data
    • Nitric Oxide Synthase
    • Shock, Septic/metabolism
    • Amyloid beta-Protein Precursor/metabolism
    • Astrocytes/metabolism
    • Astrocytes/pathology
    • Blotting, Western
    • Cerebral Cortex/metabolism
    • Cerebral Cortex/pathology
    • Cerebral Cortex/ultrastructure
    • Disease Models, Animal
    • Female
    • Glial Fibrillary Acidic Protein/metabolism
    • Immunohistochemistry
    • Microscopy, Confocal
    • Neurons/metabolism
    • Neurons/pathology
    • Neurons/ultrastructure
    • Nitric Oxide/metabolism
    • Nitric Oxide Synthase/metabolism
    • Plaque, Amyloid/metabolism
    • Plaque, Amyloid/pathology
    • Tyrosine/analogs & derivatives
    • Tyrosine/metabolism
    • Alzheimer Disease/genetics
    • Bone and Bones/abnormalities
    • Bone and Bones/embryology
    • Brain/abnormalities
    • Brain/embryology
    • Brain Chemistry/genetics
    • Gene Expression Regulation, Developmental/physiology
    • Humans
    • Male
    • Membrane Proteins/genetics
    • Mice, Knockout
    • Mutagenesis/physiology
    • Pregnancy
    • Presenilin-1
    • Transgenes/physiology
    • Amino Acid Sequence
    • Codon/genetics
    • Exons
    • Genetic Linkage
    • Genetic Variation
    • Middle Aged
    • Oligodeoxyribonucleotides
    • Pedigree
    • Point Mutation
    • Transcription, Genetic
    • Alzheimer Disease
    • Behavior, Animal
    • Brain/metabolism
    • Central Nervous System Diseases/genetics
    • Central Nervous System Diseases/mortality
    • Central Nervous System Diseases/physiopathology
    • Cosmids
    • Gene Expression
    • Genetic Vectors
    • Glucose/metabolism
    Strain Development

    Littermates were selectively bred to function as wild-type controls for MMRRC:11170 and MMRRC:11171 (see development there).

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Immunology and Inflammation
    • Models for Human Disease

    Strain Origin

    Donor
    Carl Nathan, M.D., Weill Cornell Medical College.
    Primary Reference

    Nathan C, Calingasan N, Nezezon J, Ding A, Lucia MS, La Perle K, Fuortes M,Lin M, Ehrt S, Kwon NS, Chen J, Vodovotz Y, Kipiani K, Beal MF. Protection fromAlzheimer's-like disease in the mouse by genetic ablation of inducible nitricoxide synthase. J Exp Med. 2005 Nov 7;202(9):1163-9. Epub 2005 Oct 31. (Medline PMID: 16260491)

    Hsiao K, Chapman P, Nilsen S, Eckman C, Harigaya Y, Younkin S, Yang F, Cole G.Correlative memory deficits, Abeta elevation, and amyloid plaques in transgenicmice. Science. 1996 Oct 4;274(5284):99-102. (Medline PMID: 8810256)

    MacMicking JD, Nathan C, Hom G, Chartrain N, Fletcher DS, Trumbauer M, StevensK, Xie QW, Sokol K, Hutchinson N, et al. Altered responses to bacterial infectionand endotoxic shock in mice lacking inducible nitric oxide synthase. Cell. 1995May 19;81(4):641-50. Erratum in: Cell 1995 Jun 30;81(7):following 1170. (Medline PMID: 7538909)

    Rodrigo J, Fernández-Vizarra P, Castro-Blanco S, Bentura ML, Nieto M,Gómez-Isla T, Martínez-Murillo R, MartInez A, Serrano J, Fernández AP. Nitricoxide in the cerebral cortex of amyloid-precursor protein (SW) Tg2576 transgenic mice. Neuroscience. 2004;128(1):73-89. (Medline PMID: 15450355)

    Qian S, Jiang P, Guan XM, Singh G, Trumbauer ME, Yu H, Chen HY, Van de PloegLH, Zheng H. Mutant human presenilin 1 protects presenilin 1 null mouse againstembryonic lethality and elevates Abeta1-42/43 expression. Neuron. 1998Mar;20(3):611-7. (Medline PMID: 9539133)

    Mullan M, Crawford F, Axelman K, Houlden H, Lilius L, Winblad B, Lannfelt L. Apathogenic mutation for probable Alzheimer's disease in the APP gene at theN-terminus of beta-amyloid. Nat Genet. 1992 Aug;1(5):345-7. (Medline PMID: 1302033)

    Hsiao KK, Borchelt DR, Olson K, Johannsdottir R, Kitt C, Yunis W, Xu S, EckmanC, Younkin S, Price D, et al. Age-related CNS disorder and early death intransgenic FVB/N mice overexpressing Alzheimer amyloid precursor proteins.Neuron. 1995 Nov;15(5):1203-18. (Medline PMID: 7576662)

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Breeding

    MMRRC Breeding System
    Sib-mating
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: > 12 months > 12 months
    Bred to Homozygosity
    Yes
    Average litter size
    5
    Recommended wean age
    3 weeks
    Average Pups Weaned
    5

    Order Request Information

    Availability Level

    When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    - Products for this strain are Not Yet Available for Ordering
    - If you register interest in this strain, you will be notified when it becomes available for ordering.
    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.