Strain Detail Sheet

Strain Name    :

Stock Number :

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Nos2tm1Mrl
Name: targeted mutation 1, John S Mudgett
Chromosome: 11
Alteration at locus: Knockout
Gene Symbol: Nos2
Name: nitric oxide synthase 2, inducible
Chromosome: 11
Alteration at locus: Knockout
Transgene: Tg(APPSWE)2576Kha
Name: transgene insertion 2576, Karen Hsiao Ashe
Alteration at locus: Transgenic
Transgene: APPswe (Human)
Name: amyloid beta (A4) precursor protein 695.Sweedish mutation
Alteration at locus: Transgenic
Transgene: PSEN1 (Human)
Name: presenilin 1 (Alzheimer disease 3)
Alteration at locus: Transgenic

Genetic Alterations:
For iNOS knockout, donor deleted 585bp of promoter plus exons 1-4 while inserting neo cassette (Cell 81: 641, 1995). The transgenes were introduced by others into separate strains and brought together with the ko as described above. "Recessive" designation below refers to disrupted iNOS alleles.

Genotype Determination:

  • Donor's Protocol
  • Center protocol and contact for technical support will be shipped with mice.

ES Cell Line: AB2.1 derived from C57BL/6J

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Marked amelioration of the pathology and extension of lifespan.
Hemizygous phenotype: not applicable

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6 x SJL

Strain Development: First, donor backcrossed the original iNOS-/- C57BL/6129 mice (30) to C57BL/6 for 6 generations. Descendants of brother-sister matings of the latter mice were crossed with the SJL strain and their progeny interbred to derive iNOS-/- C57BL/6SJL mice. This step was based on evidence that that modifier genes from the SJL background were critical to avoid premature mortality in C57BL/6 mice bearing a mutant (K670N, M671L) human amyloid precursor protein (APP) transgene (31). Donor bred the iNOS-/- C57BL/6SJL mice with a strain called Tg2576 in which a hamster prion promoter drives the K670N, M671L APP transgene in the C57Bl/6SJL background (32), and also with transgenics in which the Thy1 promoter drives human presenilin-1 (hPS1) with the A246E mutation in the C57Bl/6SJL background (33). Donor interbred the progeny to establish three C57BL/6SJL sublines from littermates: (i) wild type mice (iNOS+/+ hAPP0/0 hPS10/0); (ii) mice with wild type iNOS alleles and the APP and PS1 transgenes, each of which was inherited from only one parent so as to avoid overdose (iNOS+/+ hAPP+/0 hPS1+/0); and (iii) mice with disrupted iNOS alleles and the APP and PS1 transgenes (iNOS-/- hAPP+/0 hPS1+/0).

Research Applications

  • Immunology and Inflammation
  • Models for Human Disease

Strain Origin

Donor: Carl Nathan, M.D., Weill Cornell Medical College

Primary Reference:

Nathan C, Calingasan N, Nezezon J, Ding A, Lucia MS, La Perle K, Fuortes M, Lin M, Ehrt S, Kwon NS, Chen J, Vodovotz Y, Kipiani K, Beal MF. Protection from Alzheimer's-like disease in the mouse by genetic ablation of inducible nitric oxide synthase. J Exp Med. 2005 Nov 7;202(9):1163-9. Epub 2005 Oct 31. (Medline PMID: 16260491)

Colony and Husbandry Information

Special Considerations

None

Health Status Report



Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@med.unc.edu.

Appearance

Coat color:

Other:

Breeding

MMRRC Breeding System:

Breeding Scheme(s):

Generation:

Overall Breeding Performance:

Reproductive Statistics

Viability and Fertility: Unknown

Average litter size:

Recommended wean age:

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 02/26/2015