Homozygous: Not evaluated.
Heterozygous: The E391- 3608 line express E391-truncated -4R-tau, at levels that approximate those of endogenous mouse tau. The 3608 line shows lower expression than the 3610 mouse line, although it contains the same transgene. Despite lower tau expression levels, pre-tangle pathological changes in tau were observed in the 3608 mouse line to a greater extent than in the 3610 line. Both lines exhibited atypical neurites as detected by staining of antibodies to tau. Mice of the 3608 line additionally exhibited irregularly shaped dystrophic neurites with abnormal orientation to the cortical surface, similar to dystrophic neurites observed in AD. Pyramidal neurons in the cortex of 3608 mice often appeared irregular, with thickened, atypical apical dentrites. Despite these pathological changes frank tangles did not develop even though mice were aged to 2 years.
McMillan PJ, Kraemer BC, Robinson L, Leverenz JB, Raskind M, Schellenberg G. Truncation of tau at E391 Promotes Early Pathologic Changes in Transgenic Mice. J Neuropathol Exp Neurol. 2011 Nov;70(11):1006-19. (Medline PMID: 22002427).
The mice that are hemizygous for the transgene and homozygous for the targeted mutation (Mouse tau KO/KO) are mated to siblings that are transgene negative and homozygous for the targeted mutation. The mice have no unusual breeding characteristics.
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Unknown||Unknown|
|Homozygotes are fertile:||Unknown||Unknown|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Unknown||Unknown|
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to firstname.lastname@example.org for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
|MMRRC Item #||Description||Distribution Fee / Unit (US $)||Units||Notes|
|036481-JAX-SPERM||Cryo-preserved spermatozoa||$437.00 / Non-Profit||Aliquot||Approximate quantity2|
|036481-JAX-RESUS||Litter recovered from cryo-archive||$2,022.00 / Non-Profit||Litter||Recovered litter1; additional fees for any special requests.|
|Cryopreserved material may be available upon request, please inquire to email@example.com for more information.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.