Strain Name:
B6.Cg-Mapttm1Hnd Tg(Thy1-MAPT*)3608Gds/Mmjax
Stock Number:
036481-JAX
Citation ID:
RRID:MMRRC_036481-JAX
Other Names:
E391-3608, cDNA-trunc Lines 3608

Gene Information

Allele Symbol: Mapttm1Hnd (Mus musculus (mouse))
Name: microtubule-associated protein tau; targeted mutation 1, Hana N Dawson
Synonyms: TAU<->
Chromosome: 11
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:2385630
Gene Symbol: Mapt (Mus musculus (mouse))
Name: microtubule-associated protein tau
Synonyms: Tau, Mtapt
Chromosome: 11
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:97180
Promoter: Thy1 (Mus musculus (mouse))
Name: thymus cell antigen 1, theta
Synonyms: Thy-1.2, T25, Thy1.1, CD90, Thy 1.2, Thy1.2, Thy-1
Chromosome: 9
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations): MGI:98747
Transgene: MAPT (Homo sapiens (human))
Name: microtubule-associated protein tau
Chromosome: 17
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations):
Transgene: Tg(Thy1-MAPT*)3608Gds (Mus musculus (mouse))
Name: transgene insertion 3608, Gerard Schellenberg; transgene insertion 3608, Gerard Schellenberg
Synonyms: E391-3608, THY1.2::TauE391
Chromosome: UN
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations): MGI:5305850
Genetic Alterations
Human tau cDNA, truncated at glutamic acid 391(E391), was cloned into the unique XhoI site in a mouse neuron specific expression vector containing Thy 1.2 promoter.
ES Cell Line
N/A
Phenotype

Homozygous: Not evaluated.

Heterozygous: The E391- 3608 line express E391-truncated -4R-tau, at levels that approximate those of endogenous mouse tau. The 3608 line shows lower expression than the 3610 mouse line, although it contains the same transgene. Despite lower tau expression levels, pre-tangle pathological changes in tau were observed in the 3608 mouse line to a greater extent than in the 3610 line. Both lines exhibited atypical neurites as detected by staining of antibodies to tau. Mice of the 3608 line additionally exhibited irregularly shaped dystrophic neurites with abnormal orientation to the cortical surface, similar to dystrophic neurites observed in AD. Pyramidal neurons in the cortex of 3608 mice often appeared irregular, with thickened, atypical apical dentrites. Despite these pathological changes frank tangles did not develop even though mice were aged to 2 years.

Mammalian Phenotype Terms
Allelic Composition: Mapttm1Hnd/Mapttm1Hnd (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

Allelic Composition: Mapttm1Hnd/Mapt+ (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

MeSH Terms
  • Adenosine Triphosphatases/metabolism
  • Alzheimer Disease/genetics
  • Alzheimer Disease/pathology
  • Animals
  • Brain/metabolism
  • Brain/pathology
  • Cation Transport Proteins/metabolism
  • Copper-transporting ATPases
  • Disease Models, Animal
  • Gene Expression Regulation/genetics
  • Glutamic Acid/genetics
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Mutation/genetics
  • Neurons/metabolism
  • Neurons/pathology
  • Phosphorylation
  • Plaque, Amyloid/metabolism
  • Plaque, Amyloid/pathology
  • tau Proteins/genetics
  • tau Proteins/metabolism
Strain Development
The founders were backcrossed to a C57BL/6J for 12 generations. They were crossed at least once to B6.129-Mapttm1Hnd mice (mouse tau knock out line congenic with the C57BL/6J).
Suggested Control Mice
Wild-type littermates or C57BL/6J
  • Models for Human Disease
  • Neurobiology
Donor
Gerard Schellenberg, Ph.D., Perelman School of Medicine at the University of Pennsylvania
Primary Reference

McMillan PJ, Kraemer BC, Robinson L, Leverenz JB, Raskind M, Schellenberg G. Truncation of tau at E391 Promotes Early Pathologic Changes in Transgenic Mice. J Neuropathol Exp Neurol. 2011 Nov;70(11):1006-19. (Medline PMID: 22002427).

Colony and Husbandry Informationon

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • The mice that are hemizygous for the transgene and homozygous for the targeted mutation (Mouse tau KO/KO) are mated to siblings that are transgene negative and homozygous for the targeted mutation. The mice have no unusual breeding characteristics.
Generation
N13+
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036481-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity2
036481-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.