Strain Name:
D2.C3-Tg(Myh6-Ngfb)4Fed/Mmmh
Stock Number:
000121-MU
Citation ID:
RRID:MMRRC_000121-MU

Gene Information

Transgene: Ngf (Mus musculus (mouse))
Name: nerve growth factor
Synonyms: Ngfb
Chromosome: 3
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations): MGI:97321
Promoter: Myh6 (Rattus norvegicus (norway rat))
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Chromosome: 15
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations):
Transgene: Tg(Myh6-Ngfb)4Fed (Mus musculus (mouse))
Name: transgene insertion 4, Howard J Federoff; transgene insertion 4, Howard J Federoff
Synonyms: MHC-NGF, Tg(Ngfb)1Fed
Chromosome: unknown
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations): MGI:3664113
Genetic Alterations
NGF mini-gene composed of the noncodng mouse NGF exon I-intron I fused to rat NGF intron III-coding exon IV, followed by a portion of the human GH exon V and 3' UTR.
Genotype Determination
ES Cell Line
N/A
Phenotype
Cardiomegaly, sympathetic hyperinnervation
Strain Development
The alpha-MHC-NGF transgene was microinjected into the make pronucleus of fertilized C3HeB/FeJ zygotes, cultured to the two-cell stage and then implanted into oviducts of SW/Tac female mice.
Suggested Control Mice
  • Wild-type littermates
  • Cardiac pathology
Donor
Howard J. Federoff, M.D., Ph.D., University of Rochester Medical Center.
Primary Reference
Hassankhani A, Steinhelper ME, Soonpaa MH, Katz EB, Taylor DA, Andrade-Rozental A, Factor SM, Steinberg JJ, Field LJ, Federoff HJ. Overexpression of NGF within the heart of transgenic mice causes hyperinnervation, cardiac enlargement, and hyperplasia of ectopic cells. Dev Biol. 1995 May;169(1):309-21. (Medline PMID: 7750647)

Colony and Husbandry Informationon

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Agouti
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • DBA/2J female x Hemizygous transgenic male
Generation
Unknown
Overall Breeding Performance
Poor - small litter size of 3-4 pups, pre­ mature death has been occasionally observed (whencompared to their nontransgenic littermates). 
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Undetermined Undetermined
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
000121-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.