MMRRC:029795-UCD

Strain Name:

Lexicon ES cell line OST67423

Stock Number:

029795-UCD

Citation ID:

RRID:MMRRC_029795-UCD

Major Collection:

Lexicon

Gene Information Expand All

Gene Details [Including genotyping protocols]

Prdm16^{Gt(OST67423)Lex}

Name: PR domain containing 16; gene trap OST67423, Lexicon Genetics

Synonyms: Prdm16^Gt683Lex, Prdm16^LacZ

Type: Allele

Species: Mus musculus (mouse)

Chromosome: 4

Alteration at locus: Gene Trap

MGI: MGI:3531188

Genotype Determination

MMRRC's Cell Culture Protocol

ES Cell Line

Lex-1 derived from 129S5/SvEvBrd

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Strain of Origin

129/SvEvBrd

Strain Origin

Donor

Lexicon Genetics Incorporated

Primary Reference

NIH acquires knockout mice. Nature Genetics. 37:1167 (2005).
Lexicon Genetics, Inc., "NIH initiative supporting placement of Lexicon Genetics, Inc. mice into public repositories" MGI Direct Data Submission 2005;():

Colony and Husbandry Information Expand All

Special Considerations

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Health Status Report

Please view the ES cell Certificate of Analysis for this line. Contact MMRRC at UC Davis (mmrrc@ucdavis.edu) for health status information for this ES cell line.

Order Request Information Expand All

Availability Level

MMRRC testing of the Lexicon ES Cell lines includes genotyping by PCR for verification of correct target, and mycoplasma and MPV testing.

Estimated timelines: The repository was sent only one or two vials per clone/cell line from the production center. Processing ES cells (including genetic verification) prior to shipping requires 6-8 weeks.

Conditions of Distribution [Including applicable technology transfer agreements]

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires execution of the Lexicon Simple Letter Agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
029795-UCD-CELL	Lexicon ES cell line OST67423	$964.08 / Non-Profit	Vial	Includes genetic confirmation
available service	Chromosome counts	$385.17 / Non-Profit	per clone	To determine the percentage of euploid metaphase chromosomes in the targeted ES cells. At least 20 spreads are counted.
additional products	Extra vial of cells for the same clone	$462.69 / Non-Profit	per clone
additional products	Feeder cells - Irradiated	$148.67 / Non-Profit	per cell line

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet