Strain Name:
KOMP ES cell line Eif3atm1a(KOMP)Wtsi
Stock Number:
055014-UCD
Citation ID:
RRID:MMRRC_055014-UCD
Other Names:
CSD71428
Major Collection:

Gene Information

Eif3atm1a(KOMP)Wtsi
Name: eukaryotic translation initiation factor 3, subunit A; targeted mutation 1a, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout-first
Eif3a
Name: eukaryotic translation initiation factor 3, subunit A
Synonyms: A830012B05Rik, Eif3, Eif3s10, Csma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout-first
NCBI: 13669
VEGA: 19
HGNC: HGNC:3271
Homologene: 2779
Atm1Brd
Name: nonagouti; targeted mutation 1, Allan Bradley
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Targeted Mutation
Genetic Alterations

Knockout-first (Promoter-driven)

Further details at the International Mouse Phenotyping Consortium: https://www.mousephenotype.org/data/alleles/MGI:95301/tm1a%2528KOMP%2529Wtsi

KOMP Targeting Strategies

ES Cell Line
JM8A3.N1 derived from C57BL/6N
Donor
The KOMP Repository, University of California, Davis; Originating from Pieter de Jong, Kent Lloyd, William Skarnes, Allan Bradley, Wellcome Sanger Institute
Primary Reference
Skarnes, W.C., Rosen, B., West, A.P., Koutsourakis, M., Bushell, W., Iyer, V., Mujica, A.O., Thomas, M., Harrow, J., Cox, T. et al. (2011) A conditional knockout resource for the genome-wide study of mouse gene function. Nature, 474, 337-342. (PMID: 21677750)

Colony and Husbandry Information

Available Clone(s): The following clones are available for this cell line. To optimize the chances of achieving germline transmission, investigators are encouraged to order more than one clone.
  • EPD0395_4_A08
  • EPD0395_4_B05
  • EPD0395_4_C05
  • EPD0395_4_C08
  • EPD0395_4_E05
  • EPD0395_4_E06
  • EPD0395_4_E07
  • EPD0395_4_F05
  • EPD0395_4_F08
  • EPD0395_4_H06

Instructions for ordering multiple clones:

  1. Before leaving this page, you may want to copy the list of clones to paste into the special requests field as described below in Step 4.*
  2. At the bottom of this page, click "Request this Strain" button to add to your cart and proceed with the ordering process.
  3. In Step 1 of the Checkout process, select the number of additional clones desired per cell line under "Additional Clones" (ex. if ordering 3 different clones, and 1 vial of each clone, the total quantity of Additional Clones would be 2). Select any desired additional products/services for the cell line(s).
  4. In Step 4 of the Checkout process, in the "Shipping Instructions/Special Requests" box, make a note of which specific clones you are requesting.
    *Note: unless you select specific clones, the MMRRC will choose the clones.

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information

MMRRC Testing of KOMP ES Cell clones includes development of genotyping protocols, genetic verification at the 3' and 5' ends, vector copy number = 1, presence or loss of Y chromosome, and mycoplasma and MPV testing. Type of genetic testing varies for CSD and Velocigene targets. Approximately 30% of KOMP clones tested fail genetic analysis and will require a new clone be selected for expansion and testing. Based on the average germline rate for KOMP targets, we recommend requesting and injecting 2-3 clones per targeted allele. All mutant ES cell lines are heterozygous for the mutation, meaning they carry one modified allele and one wild type allele. Please note, there is high incidence of mixed cell populations in the CSD targeted cell lines, and while our testing can detect this in most cases, it is not 100%, and there may be a very small percent of non or incorrectly targeted ES cells in the population; therefore we strongly recommend targeting verification of the derived mice with 5’ or 3’ LRPCR and/or Loss of Allele analysis. MMRRC also recommends performing chromosome counts or karyotyping prior to injection of clones.

Estimated timelines: Processing ES cells (including genetic verification) prior to injection or shipping requires 12-16 weeks. Chromosome counting (to determine % euploid cells) requires an additional 4-8 weeks. Microinjection requires an additional 11-15 weeks. Germline transmission testing (breeding of chimera) requires an additional 8-16 weeks. Germline transmission is not guaranteed.

Distribution of this strain requires submission of the KOMP MTA. The MTA should be signed by an authorized official from your institution, generally this is someone in your Technology Transfer or Intellectual Property offices. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
055014-UCD-CELL KOMP ES cell line Eif3atm1a(KOMP)Wtsi $1,554.87 / $1,770.47
Non-Profit / For-Profit
Vial Includes genetic confirmation
additional products Additional Clones $718.98 / $1,022.78
Non-Profit / For-Profit
per clone
available service Chromosome counts $385.17 / $411.77
Non-Profit / For-Profit
per clone To determine the percentage of euploid metaphase chromosomes in the targeted ES cells. At least 20 spreads are counted.
additional products Extra vial of cells for the same clone $462.69 / $748.79
Non-Profit / For-Profit
per clone
available service Basic microinjection service $11,114.27 / $11,881.83
Non-Profit / For-Profit
per cell line In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed. Injection of up to 3 clones.
additional products Feeder cells - Irradiated $148.67 / $325.70
Non-Profit / For-Profit
per cell line
050961-UCD-CTRL KOMP High Passage Parental cell line JM8A3.N1 Parental Cell Sub-line (suitable for DNA extraction, control purposes; not for germline transmission) $148.67 / $306.50
Non-Profit / For-Profit
Vial Suitable for DNA extraction, control purposes but not for germline transmission.
050961-UCD-CELL KOMP Low Passage Parental cell line JM8A3.N1 Parental Cell Sub-line (suitable for electroporation and germline transmission) $740.70 / $1,441.18
Non-Profit / For-Profit
Vial Suitable for a single electroporation and germline transmission.
050961-UCD-ULTRA KOMP Ultra-low Passage Parental cell line JM8A3.N1 Parental Cell Sub-line (suitable for multiple electroporations and germline transmission) $2,059.70 / $2,993.83
Non-Profit / For-Profit
Vial Suitable for a multiple electroporation and germline transmission.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.