Register New MMRRC Account
Register for New Mouse Models
Click Here for Additional Contact Information
Availability & Fees
Order this Strain
Further details at the International Mouse Phenotyping Consortium: https://www.mousephenotype.org/data/alleles/MGI:106624/tm1a%2528KOMP%2529Wtsi
**ALERT regarding wild type JM8A3, JM8A3.N1 or JM8A1.N3 parental cells:The KOMP Project has recently identified the presence of a translocation of the manipulated agouti allele on chromosome 8 of ES cell line JM8A3, and potentially affecting the sub-lines JM8A3.N1 and JM8A1.N3, that were all derived at the Wellcome Trust Sanger Institute. At this time, we recommended not to use these cells for targeting genes on chromosome 8. Other lines, including, the JM8, JM8.N4, or JM8.F6, do not exhibit this translocation and can be safely and reliably used for gene targeting experiments. If you are using mouse lines (e.g., KOMP targeted lines) derived from JM8A3, JM8A3.N1 or JM8A1.N3 ES cell lines, we recommend crossing to C57BL/6N wild type mice in order to breed out the agouti coat color. For further information or assistance please contact Allan Bradley at The Sanger Institute email@example.com.
KOMP Targeting Strategies
Instructions for ordering multiple clones:
ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.
Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.
Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email firstname.lastname@example.org.
MMRRC Testing of KOMP ES Cell clones includes development of genotyping protocols, genetic verification at the 3' and 5' ends, vector copy number = 1, presence or loss of Y chromosome, and mycoplasma and MPV testing. Type of genetic testing varies for CSD and Velocigene targets. Approximately 30% of KOMP clones tested fail genetic analysis and will require a new clone be selected for expansion and testing. Based on the average germline rate for KOMP targets, we recommend requesting and injecting 2-3 clones per targeted allele. All mutant ES cell lines are heterozygous for the mutation, meaning they carry one modified allele and one wild type allele. Please note, there is high incidence of mixed cell populations in the CSD targeted cell lines, and while our testing can detect this in most cases, it is not 100%, and there may be a very small percent of non or incorrectly targeted ES cells in the population; therefore we strongly recommend targeting verification of the derived mice with 5’ or 3’ LRPCR and/or Loss of Allele analysis. MMRRC also recommends performing chromosome counts or karyotyping prior to injection of clones.
Estimated timelines: Processing ES cells (including genetic verification) prior to injection or shipping requires 12-16 weeks. Chromosome counting (to determine % euploid cells) requires an additional 4-8 weeks. Microinjection requires an additional 11-15 weeks. Germline transmission testing (breeding of chimera) requires an additional 8-16 weeks. Germline transmission is not guaranteed.
Distribution of this strain requires submission of the KOMP MTA. The MTA should be signed by an authorized official from your institution, generally this is someone in your Technology Transfer or Intellectual Property offices. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.