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Strain Detail Sheet

Strain Name:
EUCOMM ES cell line Ddhd2tm1e(EUCOMM)Wtsi
Stock Number:
072982-UCD
Citation ID:
RRID:MMRRC_072982-UCD
Other Names:
CSD76901
Major Collection:
The EUCOMM Collection at UC Davis

Gene Information

Gene Details

Atm1Brd
Name: nonagouti; targeted mutation 1, Allan Bradley
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Targeted Mutation
Ddhd2tm1e(EUCOMM)Wtsi
Name: DDHD domain containing 2: targeted mutation 1e
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Targeted Non-Conditional
Ddhd2
Name: DDHD domain containing 2
Synonyms: SAMWD1, 2010305K11Rik
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Targeted Non-Conditional
NCBI: 72108
Homologene: 66646
Genetic Alterations

Targeted, non-conditional (Promoter-driven)

Further details at the International Mouse Phenotyping Consortium: https://www.mousephenotype.org/data/alleles/MGI:1919358/tm1e%2528EUCOMM%2529Wtsi#esCell

**ALERT regarding wild type JM8A3, JM8A3.N1 or JM8A1.N3 parental cells:
The KOMP Project has recently identified the presence of a translocation of the manipulated agouti allele on chromosome 8 of ES cell line JM8A3, and potentially affecting the sub-lines JM8A3.N1 and JM8A1.N3, that were all derived at the Wellcome Trust Sanger Institute. At this time, we recommended not to use these cells for targeting genes on chromosome 8. Other lines, including, the JM8, JM8.N4, or JM8.F6, do not exhibit this translocation and can be safely and reliably used for gene targeting experiments. If you are using mouse lines (e.g., KOMP targeted lines) derived from JM8A3, JM8A3.N1 or JM8A1.N3 ES cell lines, we recommend crossing to C57BL/6N wild type mice in order to breed out the agouti coat color. For further information or assistance please contact Allan Bradley at The Sanger Institute abradley@sanger.ac.uk.

KOMP Targeting Strategies

ES Cell Line
JM8A3.N1 derived from C57BL/6N

Strain Description

Strain of Origin
C57BL/6N-Atm1Brd

Related Protocols

Strain Origin

Donor
Allan Bradley, Wellcome Sanger Institute, as part of the EUCOMM supported EUMMCR
Primary Reference
Skarnes, W.C., Rosen, B., West, A.P., Koutsourakis, M., Bushell, W., Iyer, V., Mujica, A.O., Thomas, M., Harrow, J., Cox, T. et al. (2011) A conditional knockout resource for the genome-wide study of mouse gene function. Nature, 474, 337-342. (PMID: 21677750)

Colony and Husbandry Information

Special Considerations

Available Clone(s): The following clones are available for this cell line. To optimize the chances of achieving germline transmission, investigators are encouraged to order more than one clone.
  • EPD0605_2_C09
  • EPD0605_2_C10
  • EPD0605_2_H12

Instructions for ordering multiple clones:

  1. Before leaving this page, you may want to copy the list of clones to paste into the special requests field as described below in Step 4.*
  2. At the bottom of this page, click "Request this Strain" button to add to your cart and proceed with the ordering process.
  3. In Step 1 of the Checkout process, select the number of additional clones desired per cell line under "Additional Clones" (ex. if ordering 3 different clones, and 1 vial of each clone, the total quantity of Additional Clones would be 2). Select any desired additional products/services for the cell line(s).
  4. In Step 4 of the Checkout process, in the "Shipping Instructions/Special Requests" box, make a note of which specific clones you are requesting.
    *Note: unless you select specific clones, the MMRRC will choose the clones.

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
072982-UCD-CELL EUCOMM ES cell line Ddhd2tm1e(EUCOMM)Wtsi $1,554.87 / Non-Profit Vial Includes genetic confirmation
available service Chromosome counts $385.17 / Non-Profit per clone To determine the percentage of euploid metaphase chromosomes in the targeted ES cells. At least 20 spreads are counted.
additional products Extra vial of cells for the same clone $462.69 / Non-Profit per clone
available service Basic microinjection service $11,114.27 / Non-Profit per cell line In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed. Injection of up to 3 clones.
additional products Feeder cells - Irradiated $148.67 / Non-Profit per cell line
050961-UCD-CTRL KOMP High Passage Parental cell line JM8A3.N1 Parental Cell Sub-line (suitable for DNA extraction, control purposes; not for germline transmission) $148.67 / $306.50
Non-Profit / For-Profit		 Vial Suitable for DNA extraction, control purposes but not for germline transmission.
050961-UCD-CELL KOMP Low Passage Parental cell line JM8A3.N1 Parental Cell Sub-line (suitable for electroporation and germline transmission) $740.70 / $1,441.18
Non-Profit / For-Profit		 Vial Suitable for a single electroporation and germline transmission.
050961-UCD-ULTRA KOMP Ultra-low Passage Parental cell line JM8A3.N1 Parental Cell Sub-line (suitable for multiple electroporations and germline transmission) $2,059.70 / $2,993.83
Non-Profit / For-Profit		 Vial Suitable for a multiple electroporation and germline transmission.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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