Strain Detail Sheet

Strain Name:
Lexicon ES cell line 2E12
Stock Number:
029669-UCD
Citation ID:
RRID:MMRRC_029669-UCD
Major Collection:
Lexicon

Gene Information

Gene Details

Htr1dtm1Lex
Name: 5-hydroxytryptamine (serotonin) receptor 1D; targeted mutation 1, Lexicon Genetics
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
Genotype Determination
MMRRC's Cell Culture Protocol
ES Cell Line
Lex-1 derived from 129S5/SvEvBrd

Strain Description

Strain of Origin
129/SvEvBrd

Related Protocols

Strain Origin

Donor
Lexicon Genetics Incorporated
Primary Reference

Colony and Husbandry Information

Special Considerations

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Health Status Report

Please view the ES cell Certificate of Analysis for this line. Contact MMRRC at UC Davis (mmrrc@ucdavis.edu) for health status information for this ES cell line.

Order Request Information

Availability Level

MMRRC testing of the Lexicon ES Cell lines includes genotyping by PCR for verification of correct target, and mycoplasma and MPV testing.

Estimated timelines: The repository was sent only one or two vials per clone/cell line from the production center. Processing ES cells (including genetic verification) prior to shipping requires 6-8 weeks.

Conditions of Distribution

Distribution of this strain requires execution of the Lexicon Simple Letter Agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
029669-UCD-CELL Lexicon ES cell line 2E12 $964.08 / Non-Profit Vial Includes genetic confirmation
available service Chromosome counts $385.17 / Non-Profit per clone To determine the percentage of euploid metaphase chromosomes in the targeted ES cells. At least 20 spreads are counted.
additional products Extra vial of cells for the same clone $462.69 / Non-Profit per clone
additional products Feeder cells - Irradiated $148.67 / Non-Profit per cell line
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.