Strain Detail Sheet

Strain Name:
C.Cg-Zfp423nur12 Amzn3129S1/SvlmJ/HamlMmucd
Stock Number:
041446-UCD
Citation ID:
RRID:MMRRC_041446-UCD

Strain Information

Gene Details

Zfp423nur12
Name: zinc finger protein 423; neurological 12
Synonyms: nurm12Jus, ataxia1
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
Zfp423
Name: zinc finger protein 423
Synonyms: Ebfaz, Roaz, Zfp104, ataxia1
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 94187
Homologene: 9010
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Amzn2
Name: anatomical modifier of Zfp423 2
Type: QTL
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chromosomal Aberration
Amzn2129S1/SvImJ
Name: 129S1/SvImJ
Type: QTL Variant
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chromosomal Aberration
Genetic Alterations
Zfp423nur12is a phenotypic mutant was identified in an ENU mutagenesis screen at the Baylor College of Medicine. A C-to-T transition mutation in the fourth exon results in the introduction of a premature stop codon. Western blot analysis showed that no detectable protein was expressed from this allele. Zfp423 is located on Chr. 8.
The quantitative trait locus Amzn3 (the anatomical modifier of Zfp423) is located on Chr. 15.
Genotype Determination
41446_Donor_GenotypingProtocol

Strain Description

Phenotype

Homozygous: Homozygous nur12 mice have ataxia and tremor and have a malformation of the cerebellum.

Heterozygous: Heterozygous mice show no nur12 phenotype.

Mammalian Phenotype Terms
Allelic Composition: Zfp423nur12/Zfp423nur12 (Genetic Background: involves: BALB/c * C57BL/6 * C3H )
Allelic Composition: Zfp423nur12/Zfp423nur12 (Genetic Background: Not Specified )
Allelic Composition: Zfp423nur12/Zfp423Gt(XH542)Byg (Genetic Background: involves: 129P2/OlaHsd * BALB/c * C57BL/6 * C3H )
MeSH Terms
  • Animals
  • Cerebellar Diseases/congenital
  • Cerebellar Diseases/genetics
  • Cerebellar Diseases/metabolism
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • Disease Models, Animal
  • Environment
  • Female
  • Genes, Modifier
  • Humans
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Transcription Factors/deficiency
  • Transcription Factors/genetics
Strain Development

Zfp423nur12 was induced on C57BL/6 and originally held on a B6 x 129 hybrid background. The line was rederived onto BALB/c. The line was crossed with 129SvIm/J to produce a congenic line containing Zfp423nur12 and chromosome 15 from 129SvIm/J.

Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor
Bruce Hamilton, Ph.D., University of California, San Diego - School of Medicine.
Primary Reference

Alcaraz WA, Chen E, Valdes P, Kim E, Lo YH, Vo J, Hamilton BA. Modifier genes and non-genetic factors reshape anatomical deficits in Zfp423-deficient mice. Hum Mol Genet. 2011 Oct 1;20(19):3822-30. (Medline PMID: 21729880)

Colony and Husbandry Information

Special Considerations

Homozygous nur12 mutants may require wet food and easily accessible water.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
White

Breeding

MMRRC Breeding System
Backcross
Generation
N27
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months 8 to 12 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.