Strain Name:
B6J.NOD-Idd5NOD/ShiLtJ Idd4.3NOD/ShiLtJ/LithMmjax
Stock Number:
069567-JAX
Citation ID:
RRID:MMRRC_069567-JAX
Other Names:

B6.NODC11xC1tb, B6J.NOD-(D1Mit5.1-D1Mit15) (Csf2-D11Mit42)/LithMmjax

Gene Information

(D1Mit5.1-D1Mit15)
Type: Allele
Species: Mus musculus (mouse)
(Csf2-D11Mit42)
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
D1Mit5.1
Name: DNA segment, Chr 1, Massachusetts Institute of Technology 5.1
Synonyms: D1Mit5
Type: DNA Segment
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chromosomal Aberration
D1Mit15
Name: DNA segment, Chr 1, Massachusetts Institute of Technology 15
Type: DNA Segment
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chromosomal Aberration
D11Mit42
Name: DNA segment, Chr 11, Massachusetts Institute of Technology 42
Type: DNA Segment
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chromosomal Aberration
Csf2
Name: colony stimulating factor 2 (granulocyte-macrophage)
Synonyms: Gm-CSf, Csfgm, MGI-IGM, GMCSF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chromosomal Aberration
NCBI: 12981
HGNC: HGNC:2434
Homologene: 600
Idd5
Name: insulin dependent diabetes susceptibility 5
Synonyms: Idd-5
Type: QTL
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chromosomal Aberration
Idd4.3
Name: insulin dependent diabetes susceptibility 4.3
Synonyms: Idd4.3, Idd4c
Type: QTL
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chromosomal Aberration
Genetic Alterations
This B6J-congenic strain carries epigenetic variant loci on Chr11 (enhancer for CSF2 gene) and Chr1 (enhancer for Ptgs2 gene).
ES Cell Line
Not applicable
Phenotype
Pancreatic islet immune cell infiltration. Diabetes-prone though delayed compared to NOD parent. High expression of GM-CSF and COX2. Constitutive STAT5 tyrosine phosphorylation.

Note: Only frozen samples are available. The epigenetic phenotype will be lost if surrogate-implanted, due to maternal in utero alterations of epigenetic mutations. The line requires congenic selection with C57BL/6j background breeding.
Strain Development
Crossed lines carrying separate alleles to B6J congenicity.
Suggested Control Mice
C57BL/6J
  • Diabetes
  • Immunology and Inflammation
  • Models for Human Disease
Donor
Sally Litherland, Ph.D., AdventHealth Cancer Institute.
Primary Reference

Garrigan E, Belkin NS, Seydel F, Han Z, Carter J, McDuffie M, Morel L, Peck AB, Clare-Salzler MJ, Atkinson M, Wasserfall C, Davoodi-Semiromi A, Shi JD, Haskell-Luevano C, Yang LJ, Alexander JJ, Cdebaca A, Piliant T, Riggs C, Amick M, Litherland SA. Csf2 and Ptgs2 Epigenetic Dysregulation in Diabetes-prone Bicongenic B6.NODC11bxC1tb Mice. Genet Epigenet. 2015 Oct 11;7:5-17. doi: 10.4137/GEG.S29696. (Medline PMID: 26512207)

Colony and Husbandry Information

The donor recommends monitoring for hyperglycemia after 8 weeks. The mice can develop insulitis and diabetes at later ages (16-30 wks).
For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Black
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Generation
was at least 6 at sperm cryopreservation
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4 to 6
Recommended wean age
4 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069567-JAX-HOM-F
069567-JAX-HOM-M
Homozygous Female
Homozygous Male
$218.00 / $218.00
Non-Profit / For-Profit
Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.