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Strain Detail Sheet
Strain Name:
Izon ES cell line Sh3gl2
Stock Number:
010599-UCD
Citation ID:
RRID:MMRRC_010599-UCD
Sh3gl2
Name: SH3-domain GRB2-like 2
Synonyms: EEN-B1, endophilin I, Sh3d2a, 9530001L19Rik, B930049H17Rik, endophilin A1, EEN1
Type: Gene
Species: Mouse
Chromosome: 4
MGI: MGI:700009
NCBI: 20404
Ensembl: ENSMUSG00000028488
VEGA: OTTMUSG00000007959
HGNC: HGNC:10831
Homologene: 20652
CCDS: CCDS18303.1
UCSC: Chr4:85123663-85307617
Genetic Alterations
Neo cassette (floxed) inserted into exon 8, which should result in a frame shift (loss of SH3 domain)
ES Cell Line
Bruce4 derived from B6.PL-Thy1a/CyJ
Strain of Origin
B6.Cg-Thy1a/CyJ
Donor
David Izon, Ph.D., Telethon Institute for Child Health Research
ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Resequencing adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the
MMRRC Catalog Search to request more than one strain.)
| MMRRC Item # | Description | Distribution Fee / Unit (US $) *Shipping & Handling not included* |
Units | Notes |
|---|---|---|---|---|
| 010599-UCD-CELL | Izon ES cell line Sh3gl2 | $1,192.87 / $1,192.87 Non-Profit / For-Profit |
Vial | Includes genetic confirmation |
| available service | Chromosome counts | $385.17 / $411.77 Non-Profit / For-Profit |
per clone | To determine the percentage of euploid metaphase chromosomes in the targeted ES cells. At least 20 spreads are counted. |
| additional products | Extra vial of cells for the same clone | $462.69 / $748.79 Non-Profit / For-Profit |
per clone | |
| available service | Basic microinjection service | $11,114.27 / $11,881.83 Non-Profit / For-Profit |
per cell line | In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed. Injection of up to 3 clones. |
| additional products | Feeder cells - Irradiated | $148.67 / $325.70 Non-Profit / For-Profit |
per cell line |
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the
Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.