Strain Detail Sheet

Strain Name:

Stock Number:

Other Names:

Gene Information

Gene Detail

Alteration at locus: Non-mutant

Genetic Alterations:

Genotype Determination:

Parental ES Cell Line: Nagy ES cell line R1 (MMRRC:011978)

Cell line development:
This is a subclone of the bulk R1 ES cell line (MMRRC:011978) and has better developmental potential when tested by tetraploid complement assay and is superior to the R1 ES cell line.
Mouse strain from which the parental cell line was derived: Hybrid of two 129 substrains (129X1/SvJ and 129S1/SV-+p+Tyr-cKitlSl-J/+)

Related Protocols

Strain Origin

Donor: Andras Nagy, Ph.D., Mt. Sinai Hospital, Toronto

Primary Reference:

Nagy A, Rossant J, Nagy R, Abramow-Newerly W, Roder JC. Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8424-8. (Medline PMID: 8378314 )

Special Considerations

The Donating Investigator reports that this subclone of bulk R1 ES cells exhibits improved developmental potential compared to the standard R1 ES cell line.

Supplemental Information

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email .

Order Request Information

Availability Level

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes

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To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-7510 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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