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Strain Detail Sheet

Strain Name:
Nagy ES cell line R1 with EGFP (D4)
Stock Number:
011980-MU
Citation ID:
RRID:MMRRC_011980-MU
Major Collection:
Nagy Basic ES Cell lines

Gene Information

Gene Details

EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
ACTB
Name: actin, beta
Type: Gene
Species: Gallus gallus (chicken)
Chromosome: 14
CMV
Name: cytomegalovirus
Type: Regulatory Region
Species: Virus
Chromosome:
Alteration at locus: Transgenic
MGI:
Genetic Alterations
Insertion site is located on the X-chromosome.
Cell line contains the EGFP transgene under control of the CMV immediate early enhancer coupled to the chicken -actin promoter.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+

Related Protocols

Origin

Donor
Andras Nagy, Ph.D., Mt. Sinai Hospital, Toronto
Primary Reference

Hadjantonakis AK, Gertsenstein M, Ikawa M, Okabe M, Nagy A. Generating green fluorescent mice by germline transmission of green fluorescent ES cells. Mech Dev. 1998 Aug;76(1-2):79-90. (Medline PMID: 9867352)

Additional Information on the R1 ES cell line

Additional Information

Special Considerations

Transgenic mice (STOCK Tg(ACTB-EGFP)D4Nagy/J) have been made from this cell line.

Health Status Report

This ES cell line has been tested and found to be free of Mycoplasma sp., MPV and MVM. Please contact MU Technical Services at mmrrc@missouri.edu or at (888) 673-6459 if you would like to order additional health testing.

Order Information

Availability Level

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The submitter or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
011980-MU-CELL Nagy ES cell line R1 with EGFP (D4) $546.00 / Non-Profit Vial Includes genetic confirmation
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.