Strain Detail Sheet

Strain Name:

Nagy ES cell line G4 with DsRed.MST transgene

Stock Number:

011988-MU

Other Names:

Gene Information

Gene Detail

Enhancer: CMV (Viral)
Name: cytomegalovirus
Alteration at locus: Transgenic
Promoter: ACTB (Chicken)
Name: actin, beta
Alteration at locus: Transgenic
Reporter: Dsred.MST (Sea anemone)
Name: Red Fluorescent Protein gene
Alteration at locus: Transgenic

Genetic Alterations:
ES cell lines containing a transgenic construct consisting of a Red Fluorescent Protein variant gene under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene (MMRRC:011987) were transiently exposed to cre-recombinase which excised the beta-geo gene leading to expression of the DsRed.MST transgene.

Genotype Determination:

Parental ES Cell Line: Nagy ES cell line G4 with Z/red transgene (MMRRC:011987)
Mouse strain from which the parental cell line was derived: 129S6/SvEvTac X C57BL/6Ncr F1

Related MMRRC Products:
ACTB: MMRRC:011981-MU Nagy ES cell line R1 with EGFP (B5)
ACTB: MMRRC:011982-MU Nagy ES cell line R1 with EYFP (YC5)
ACTB: MMRRC:011983-MU Nagy ES cell line R1 with EYFP (7AC5)
ACTB: MMRRC:011984-MU Nagy ES cell line R1 with ECFP (CK6)
ACTB: MMRRC:011985-MU Nagy ES cell line R1 with ECFP (CK8)
ACTB: MMRRC:011987-MU Nagy ES cell line G4 with Z/red transgene
ACTB: MMRRC:011991-MU Nagy ES cell line YV1
ACTB: MMRRC:036801-UNC STOCK Tg(ACTB-HistH2b/GFP)1Mboi/Mmnc

Related Protocols

Strain Origin

Donor: Andras Nagy, Ph.D., Mt. Sinai Hospital, Toronto

Primary Reference:

  • Vintersten K, Monetti C, Gertsenstein M, Zhang P, Laszlo L, Biechele S, Nagy A. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis. 2004 Dec;40(4):241-6. Erratum in: Genesis. 2005 Jul;42(3):218. (Medline PMID: 15593332)
  • George SH, Gertsenstein M, Vintersten K, Korets-Smith E, Murphy J, Stevens ME, Haigh JJ, Nagy A. Developmental and adult phenotyping directly from mutant embryonic stem cells. Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4455-60. Epub 2007 Mar 2. (Medline PMID: 17360545)

Special Considerations

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
011988-MU-CELLNagy Basic ES cell line G4 with DsRed.MST transgene
$546.00
Non-Profit
Vial Without genetic confirmation.*

*Genetic confirmation of a cell line is an add-on service; cost is in addition to the cost of the cell line itself. We recommend minimum verification of the cell line by genetic confirmation of the reporter sequence. Please inquire with the supplying MMRRC facility when ordering (mmrrc@missouri.edu).

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-7510 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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