ES cell lines containing a transgenic construct consisting of a Red Fluorescent Protein variant gene under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene (MMRRC:011987) were transiently exposed to cre-recombinase which excised the beta-geo gene leading to expression of the DsRed.MST transgene.
Donor: Andras Nagy, Ph.D., Mt. Sinai Hospital, Toronto
Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email firstname.lastname@example.org.
Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
|MMRRC Item #||Description||Distribution Fee / Unit (US $)||Units||Notes|
|011988-MU-CELL||Nagy Basic ES cell line G4 with DsRed.MST transgene||$546.00 / Non-Profit||Vial||Without genetic confirmation.*|
*Genetic confirmation of a cell line is an add-on service; cost is in addition to the cost of the cell line itself. We recommend minimum verification of the cell line by genetic confirmation of the reporter sequence. Please inquire with the supplying MMRRC facility when ordering (email@example.com).