Strain Detail Sheet

Strain Name:
Parental ES cell line C2
Stock Number:
011989-MU
Citation ID:
RRID:MMRRC_011989-MU
Major Collection:
Nagy Basic ES Cell lines

Gene Information

Gene Details

Genetic Alterations
Non-mutant, there is no genetic alteration.

Strain Description

Strain of Origin
C57BL/6NTac

Related Protocols

Strain Origin

Donor
Andras Nagy, Ph.D., Mt. Sinai Hospital, Toronto
Primary Reference
Not published but the following information was provided by the donating investigators laboratory:

  • C2 and D4 lines were derived during the same experiment in 1999. These two lines had best chromosome count among others and were determined XY by PCR at that time (which does not preclude them from loosing Y chromosome during in vitro culture). Both C2 and D4 aggregated at early passage with outbred ICR morulae and resulted in chimeras that did not, however, transmit through germline.
  • Both lines were tested for chimera generation before any genetic manipulations by a collaborator who found that using injections with BALB/c blastocysts produced germline chimeras. Prolonged in vitro culture during gene targeting found D4 to be more susceptible to differentiation than C2. This collaborator has now used C2 for quite a few years and has satisfactory recombination and generation of chimeras with BALB/c blastocysts.
  • Donor did not test generation of chimeras using BALB/c or albino C57BL/6 host embryos in his lab. Donor subcloned both C2 and D4 lines in TX medium manufactured by Belgian company Thromb-X (now distributed by Chemicon under the name of RESGRO). After subcloning in TX medium, both lines were capable of generating germline transmitting chimeras after aggregations with ICR embryos.
  • Donor performed one electroporation in his lab using the C2 line, two subclones with randomly integrated transgene (EGFP) have produced germline transmitting chimeras after aggregations with ICR embryos. TX-subcloned pool of C2 were used for electroporation in regular medium. After identifying good expressors, cells were grown in TX-medium for two passages before aggregations.

Colony and Husbandry Information

Special Considerations

Health Status Report

This ES cell line has been tested and found to be free of Mycoplasma sp., MPV and MVM. Please contact MU Technical Services at mmrrc@missouri.edu or at (888) 673-6459 if you would like to order additional health testing.

Order Request Information

Availability Level

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
011989-MU-CELL Parental ES cell line C2 $546.00 / Non-Profit Vial Includes genetic confirmation
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.