Strain Detail Sheet

Strain Name:

E14 RP11-88L12 NKX2-5-EmGFP ES cell line

Stock Number:

030473-UCD

Other Names:

Gene Information

Gene Detail

Reporter: EmGFP (Jelly Fish)
Name: Emerald Green Fluorescent Protein
Alteration at locus: Transgenic (BAC)
Transgene: NKX2-5 (Human)
Name: NK2 transcription factor related, locus 5
Alteration at locus: Transgenic (BAC)

Genetic Alterations:
This is an ES cell line, drived from the E14Tg2A.4 mouse ES cell line. Mouse E14 ES cells were electroporated with a modified human BAC for NKX2-5-EmGFP to make the transgenic reporter line. The BAC is based on RP11-88L12, and carries an in-frame P2A-EmGFP insertion, a Zeocin cassette, and a SV40- Neo selection cassette.

Genotype Determination:

Parental ES Cell Line: E14Tg2A.4
Mouse strain from which the parental cell line was derived: 129P2/OlaHsd

Related MMRRC Products:

Related Protocols

Strain Origin

Donor: Bruce Conklin, M.D., Gladstone Institute of Cardiovascular Disease

Primary Reference:

Hsiao EC, Yoshinaga Y, Nguyen TD, Musone SL, Kim JE, Swinton P, Espineda I, Manalac C, deJong PJ, Conklin BR. Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter. PLoS ONE. 2008 Jul 2;3(7):e2532. (Medline PMID: 18596956)

Special Considerations

This is Clone #2 as described in publication. See also: MMRRC:030440

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
030473-UCD-CELL E14 RP11-88L12 NKX2-5-EmGFP
$546.00
Non-Profit
Vial

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-7510 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.