Strain Detail Sheet

Strain Name:
E14 RP11-88L12 NKX2-5-EmGFP ES cell line
Stock Number:
030473-UCD
Citation ID:
RRID:MMRRC_030473-UCD

Gene Information

Gene Details

NKX2-5
Name: NK2 transcription factor related, locus 5
Type: Gene
Species: Homo sapiens (human)
Chromosome: 5
EmGFP
Name: emerald green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Genetic Alterations
This is an ES cell line, derived from the E14Tg2A.4 mouse ES cell line. Mouse E14 ES cells were electroporated with a modified human BAC for NKX2-5-EmGFP to make the transgenic reporter line. The BAC is based on RP11-88L12, and carries an in-frame P2A-EmGFP insertion, a Zeocin cassette, and a SV40- Neo selection cassette.
Genotype Determination

Genotyping Protocol

Media protocol (for both culture and differentiation protocols)

Culture protocol

Cardiomyocyte Differentiation protocol

ES Cell Line
E14TG2a.4 derived from 129P2/OlaHsd

Related Protocols

Strain Origin

Donor
Bruce Conklin, M.D., Gladstone Institute of Cardiovascular Disease
Primary Reference
Hsiao EC, Yoshinaga Y, Nguyen TD, Musone SL, Kim JE, Swinton P, Espineda I, Manalac C, deJong PJ, Conklin BR. Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter. PLoS ONE. 2008 Jul 2;3(7):e2532. (Medline PMID: 18596956)

Colony and Husbandry Information

Special Considerations

This is Clone #2 as described in publication. See also: MMRRC:030440

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Cryo-preserved ES cell line, material can usually be shipped in 2 to 4 weeks.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
030473-UCD-CELL E14 RP11-88L12 NKX2-5-EmGFP ES cell line $1,192.87 / Non-Profit Vial Includes genetic confirmation
additional products Extra vial of cells for the same clone $462.69 / Non-Profit per clone
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.