This is an ES cell line, drived from the E14Tg2A.4 mouse ES cell line. Mouse E14 ES cells were electroporated with a modified human BAC for NKX2-5-EmGFP to make the transgenic reporter line. The BAC is based on RP11-88L12, and carries an in-frame P2A-EmGFP insertion, a Zeocin cassette, and a SV40- Neo selection cassette.
Donor: Bruce Conklin, M.D., Gladstone Institute of Cardiovascular Disease
Hsiao EC, Yoshinaga Y, Nguyen TD, Musone SL, Kim JE, Swinton P, Espineda I, Manalac C, deJong PJ, Conklin BR. Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter. PLoS ONE. 2008 Jul 2;3(7):e2532. (Medline PMID: 18596956)
Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email firstname.lastname@example.org.
Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
|MMRRC Item #||Description||Distribution Fee / Unit (US $)||Units||Notes|
|030473-UCD-CELL||E14 RP11-88L12 NKX2-5-EmGFP||$682.50 / Non-Profit||Vial||Without genetic confirmation.*|