Strain Detail Sheet

Strain Name:
Soriano Gene Trap ES cell line S16-10A1_A10
Stock Number:
031260-UCD
Citation ID:
RRID:MMRRC_031260-UCD
Major Collection:
Soriano Gene Trap Lines

Gene Information

Gene Details

Map1b
Name: microtubule-associated protein 1B
Synonyms: MAP5, Mtap-5, Mtap5, LC1, Mtap1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17755
VEGA: 13
HGNC: HGNC:6836
Homologene: 38111
Genetic Alterations
Disruption caused by insertion of the retroviral gene trap vector ROSAFARY.
Genotype Determination
ES Cell Line
AK7.1 (MMRRC Stock Number 015891-UCD)
IGTC Cell Line Annotation

Related Protocols

Strain Origin

Donor
Soriano, Philippe, Mount Sinai Medical Center, NY (See Soriano Lab Overview)
Primary Reference
Friedrich G, Soriano P., Insertional mutagenesis by retroviruses and promoter traps in embryonic stem cells. Methods Enzymol 1993;225:681-701.(Medline PMID: 8231879 )
Chen WV, Soriano P. Gene trap mutagenesis in embryonic stem cells. Methods Enzymol. 2003;365:367-86. (Medline PMID: 14696359)

Colony and Husbandry Information

Special Considerations

When choosing a clone, we recommend blasting the sequence tag to estimate the location of a gene trap. Generally you will want to select a clone that traps the furthest upstream. The vast majority of gene trap cell lines have been correctly identified, as judged by DNA sequencing (i.e., repeat determination of the DNA sequence tag when the ES cell clone is requested). However, incorrectly identified ES cell clones are occasionally encountered, either because of human error or because a thawed ES cell line gets overgrown by a contaminating ES cell line. Therefore, we strongly recommend that you request verification of the identity of your ES cell line by DNA sequencing (there is an additional fee for DNA sequence verification of your clone). We also recommend that recipients of ES cells re-verify the identity of the ES cell line by RT-PCR and/or Southern blot analysis. If more than one ES cell line is available for your "gene-of-interest", we recommend that you order several of the ES cell lines. Ordering more than one ES cell line for a specific 'gene-of-interest' will increase the chances of success.

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. The parental cell line K7.1, from which these cells were derived, was tested for multiple mouse pathogens; please see the Health Status Report.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

MMRRC Testing of the BayGenomics, SIGTR, and Soriano gene trap cell lines includes RACE sequencing and mycoplasma and MPV testing. All mutant ES cell lines are heterozygous for the mutation, meaning they carry one modified allele and one wild type allele. MMRRC also recommends performing chromosome counts or karyotyping prior to injection of clones.

Estimated timelines: The repository was sent only one or two vials per clone/cell line from the production center. Processing ES cells (including genetic verification) prior to shipping or injection requires 6-8 weeks. Chromosome counting (to determine % euploid cells) requires an additional 4-8 weeks. Microinjection requires an additional 11-15 weeks. Germline transmission testing (breeding of chimera) requires an additional 8-16 weeks. Germline transmission is not guaranteed.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
031260-UCD-CELL Soriano Gene Trap ES cell line S16-10A1_A10 $1,192.87 / $1,192.87
Non-Profit / For-Profit		 Vial Includes genetic confirmation
available service Chromosome counts $385.17 / $411.77
Non-Profit / For-Profit		 per clone To determine the percentage of euploid metaphase chromosomes in the targeted ES cells. At least 20 spreads are counted.
additional products Extra vial of cells for the same clone $462.69 / $748.79
Non-Profit / For-Profit		 per clone
available service Basic microinjection service $11,114.27 / $11,881.83
Non-Profit / For-Profit		 per cell line In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed. Injection of up to 3 clones.
015891-UCD-CTRL Soriano Gene Trap Lines High Passage Parental cell line Soriano ES Parental cell line AK7 (suitable for DNA extraction, control purposes; not for germline transmission) $148.67 / $306.50
Non-Profit / For-Profit		 Vial Suitable for DNA extraction, control purposes but not for germline transmission.
015891-UCD-CELL Soriano Gene Trap Lines Low Passage Parental cell line Soriano ES Parental cell line AK7 (suitable for electroporation and germline transmission) $740.70 / $1,441.18
Non-Profit / For-Profit		 Vial Suitable for a single electroporation and germline transmission.
015891-UCD-ULTRA Soriano Gene Trap Lines Ultra-low Passage Parental cell line Soriano ES Parental cell line AK7 (suitable for multiple electroporations and germline transmission) $2,059.70 / $2,993.83
Non-Profit / For-Profit		 Vial Suitable for a multiple electroporation and germline transmission.
015892-UCD-CELL Include vial of feeder line SNL 76/7 STO $112.00 / $277.87
Non-Profit / For-Profit		 Vial In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.