Strain Detail Sheet

Strain Name:

Conklin ES cell line R26(EF1α-tTA/TetO-mCh-Rs1)

Stock Number:

034358-MU

Other Names:

Gene Information

Gene Detail

Target: Gt(ROSA)26Sortm1(Eif1a-tTA,tetO-mCherry/HTR4*D100A)Conk
Name:
Alteration at locus: Knock-In

Genetic Alterations:
E14Tg2A.4 mouse embryonic stem cells were electroporated with a Rosa26 vector carrying the combined EF1a-tTA and TetO-mCh-Rs1 transgenes in a single construct. ES cells remain on the 129P2/OlaHsd background.

Genotype Determination:

Parental ES Cell Line: E14Tg2A.4
Mouse strain from which the parental cell line was derived: 129P2/OlaHsd

Related MMRRC Products:

Related Protocols

Strain Origin

Donor: Bruce Conklin, M.D., Gladstone Institute of Cardiovascular Disease

Primary Reference:

Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR. Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice.  Stem Cell Res Ther. 2011 Mar 4;2(2):11. (Medline PMID: 21375737).

Special Considerations

Donor recommends maintining the ES cell line in doxy-containing media to prevent any unexpected effects from Rs1 expression.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
034358-MU-CELLConklin ES cell line R26(EF1α-tTA/TetO-mCh-Rs1)
$546.00
Non-Profit
Vial Without genetic confirmation.*

*Genetic confirmation of a cell line is an add-on service; cost is in addition to the cost of the cell line itself. We recommend minimum verification of the cell line by genetic confirmation of the reporter sequence. Please inquire with the supplying MMRRC facility when ordering (mmrrc@missouri.edu).

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-7510 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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