Strain Detail Sheet

Strain Name:
Conklin ES cell line R26(EF1α-tTA/TetO-mCh-Rs1)
Stock Number:
034358-MU
Citation ID:
RRID:MMRRC_034358-MU

Gene Information

Gene Details

Gt(ROSA)26Sortm1(Eif1a-tTA,tetO-mCherry/HTR4*D100A)Conk
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Bruce Conklin
Synonyms: R26(EF1a-tTA/TetO-mCh-Rs1), TetO-mCh-Rs1
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
Genetic Alterations
E14Tg2A.4 mouse embryonic stem cells were electroporated with a Rosa26 vector carrying the combined EF1a-tTA and TetO-mCh-Rs1 transgenes in a single construct. ES cells remain on the 129P2/OlaHsd background.
Genotype Determination

ES Cell Line
E14TG2a.4 derived from 129P2/OlaHsd

Strain Description

Strain of Origin
129P2/OlaHsd

Related Protocols

Strain Origin

Donor
Bruce Conklin, M.D., Gladstone Institute of Cardiovascular Disease
Primary Reference
Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR. Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice.  Stem Cell Res Ther. 2011 Mar 4;2(2):11. (Medline PMID: 21375737).

Colony and Husbandry Information

Special Considerations

Donor recommends maintining the ES cell line in doxy-containing media to prevent any unexpected effects from Rs1 expression.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@missouri.edu.

Order Request Information

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
034358-MU-CELL Conklin ES cell line R26(EF1α-tTA/TetO-mCh-Rs1) $546.00 / Non-Profit Vial Includes genetic confirmation
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.