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Strain Detail Sheet

Strain Name:
Pleiades Promoter Project ES cell line mEMS3986
Stock Number:
035797-JAX
Citation ID:
RRID:MMRRC_035797-JAX
Major Collection:
Pleiades Promoter Project

Gene Information

Gene Details

Hprt1
Name: hypoxanthine phosphoribosyltransferase 1
Synonyms: Hprt1, Hprt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 15452
HGNC: HGNC:5157
Homologene: 56590
lacZ
Name: beta galactosidase
Type: Gene
Species: Escherichia coli (bacterium)
Chromosome:
ADORA2A
Name: adenosine A2a receptor
Type: Gene
Species: Homo sapiens (human)
Chromosome: 22
ES Cell Line
mEMS1202 derived from (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1

Strain Origin

Donor
Elizabeth M. Simpson, University of British Columbia.
Primary Reference
Portales-Casamar E; Swanson DJ; Liu L; de Leeuw CN; Banks KG; Ho Sui SJ; Fulton DL; Ali J; Amirabbasi M; Arenillas DJ; Babyak N; Black SF;onaguro RJ; Brauer E; Candido TR; Castellarin M; Chen J; Chen Y; Cheng JC; Chopra V; Docking TR; Dreolini L; D'Souza CA; Flynn EK; Glenn R; Hatakka K; Hearty TG; Imanian B; Jiang S; Khorasan-zadeh S; Komljenovic I; Laprise S; Liao NY; Lim JS; Lithwick S; Liu F; Liu J; Lu M; McConechy M; McLeod AJ; Milisavljevic M; Mis J; O'Connor K; Palma B; Palmquist DL; Schmouth JF; Swanson MI; Tam B; Ticoll A; Turner JL; Varhol R; Vermeulen J; Watkins RF; Wilson G; Wong BK; Wong SH; Wong TY; Yang GS; Ypsilanti AR; Jones SJ; Holt RA; Goldowitz D; Wasserman WW; Simpson EM. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A 2010 Sep 21;107(38):16589-94 (Medline PMID: 20807748)

Colony and Husbandry Information

Special Considerations

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Health Status Report

Prior to shipping, the requested cell lines are tested for specific pathogens such as Mycoplasma spp., Mouse Parvoviruses (MPV), and many others. Additional health status information may be available upon request. Please inquire with the supplying MMRRC facility when ordering (csmmrrc@jax.org).

Order Request Information

Availability Level

Additional services, such as genetic confirmation, may add to the time of shipping. Please inquire with the supplying MMRRC facility when ordering csmmrrc@jax.org.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
035797-JAX-CELL Pleiades Promoter Project ES cell line mEMS3986 $546.00 / Non-Profit Vial Includes genetic confirmation
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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