• Genotyping Protocol
• Donor's Protocols

• Donor's Protocols
• miRKO Database information available such as vector design, PCR results, and gene details
• Mirbase.org searchable database of published miRNA sequences and annotation
• EBI MicroCosm Targets contains computationally predicted targets for microRNAs
• Michael McManus' Lab

• miR-146a-3

Instructions for ordering multiple clones:

1. Before leaving this page, you may want to copy the list of clones to paste into the special requests field as described below in Step 4.*
2. At the bottom of this page, click "Request this Strain" button to add to your cart and proceed with the ordering process.
3. In Step 1 of the Checkout process, select the number of additional clones desired per cell line under "Additional Clones" (ex. if ordering 3 different clones, and 1 vial of each clone, the total quantity of Additional Clones would be 2). Select any desired additional products/services for the cell line(s).
4. In Step 4 of the Checkout process, in the "Shipping Instructions/Special Requests" box, make a note of which specific clones you are requesting.
   *Note: unless you select specific clones, the MMRRC will choose the clones.

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

MMRRC Testing of the Sanger and Keck MirKO cell lines includes genetic verification at the 3' and/or 5' ends, vector copy number = 1, presence or loss of Y chromosome, and mycoplasma and MPV testing. Based on the average germline rates, we recommend requesting and injecting 2-3 clones per targeted allele. All mutant ES cell lines are heterozygous for the mutation, meaning they carry one modified allele and one wild type allele. MMRRC also recommends performing chromosome counts or karyotyping prior to injection of clones.

Estimated timelines: The repository was sent only one or two vials per clone/cell line from the production center. Processing ES cells (including genetic verification) prior to shipping or injection requires 6-8 weeks. Chromosome counting (to determine % euploid cells) requires an additional 4-8 weeks. Microinjection requires an additional 11-15 weeks. Germline transmission testing (breeding of chimera) requires an additional 8-16 weeks. Germline transmission is not guaranteed.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.