Strain Name:
Keck MirKO ES cell line Mir146a
Stock Number:
036857-UCD
Citation ID:
RRID:MMRRC_036857-UCD
Other Names:
miR-146a
Major Collection:

Gene Information

Gene Symbol: Mir146 (Mus musculus (mouse))
Name: microRNA 146
Chromosome: 11
Alteration at locus: Knockout-first
Monarch Initiative (Disease and Phenotype Associations): MGI:2676831
Genetic Alterations
A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the miR stem loop, with one loxP site placed immediately downstream of the miR stem loop.
Genotype Determination
ES Cell Line
E14TG2a.4 derived from 129P2/OlaHsd
Donor
Michael McManus, Ph.D., University of California, San Francisco, W.M.Keck Center for Noncoding RNAs
Primary Reference
Park CY, Jeker LT, Carver-Moore K, Oh A, Liu HJ, Cameron R, Richards H, Li Z, Adler D, Yoshinaga Y, Martinez M, Nefadov M, Abbas AK, Weiss A, Lanier LL, de Jong PJ, Bluestone JA, Srivastava D, McManus MT. A Resource for the Conditional Ablation of microRNAs in the Mouse. Cell Rep. 2012 Apr 19;1(4):385-91. (Medline PMID: 22570807).

Colony and Husbandry Information

Available Clone(s): The following clones are available for this cell line. To optimize the chances of achieving germline transmission, investigators are encouraged to order more than one clone.
  • miR-146a-3

Instructions for ordering multiple clones:

  1. Before leaving this page, you may want to copy the list to paste into the special notes field as described below in the last step.
  2. At the bottom of this page, click "Request this Strain" button to access the MMRRC order form.
  3. In Step 1 under "Quantity" enter the total number of vials desired per cell line (ex. if ordering 3 different clones, and 1 vial of each clone, the total quantity would be 3).
  4. In Step 4 in the "Shipping Instructions/ special notes" box make a note of how many different clones you will be requesting*.
    *Note: unless otherwise specified, the MMRRC will choose the specific clones

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks.Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036857-UCD-CELL Keck MirKO ES cell line miR-146a $682.50 / Non-Profit Vial Without genetic confirmation.*
recommended service Mutant ES cell line genetic confirmation service $414.75 / Non-Profit per cell line Genetic confirmation recommended. *Service fee is in addition to fee for mutant cell line.
available service Basic microinjection service $7,396.94 / Non-Profit per cell line In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed.

*Genetic confirmation of a cell line is an add-on service; cost is in addition to the cost of the cell line itself. We recommend minimum verification of the cell line by genetic confirmation (see MMRRC ES Cell Clones and Related Cell Lines for additional information).

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.