MMRRC:050957-UCD

Strain Name:

JM8 Parental Cell Line

Stock Number:

050957-UCD

Citation ID:

RRID:MMRRC_050957-UCD

Other Names:

JM8 Parental Cell Line

Major Collection:

KOMP

Gene Information Expand All

Gene Details [Including genotyping protocols]

ES Cell Line

JM8 derived from C57BL/6N

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Strain of Origin

C57BL/6N

Strain Development

These ES Cells were derived from C57BL/6N mice.

Strain Origin

Donor

The KOMP Repository , UC Davis Mouse Biology Program.

Primary Reference

Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM, Beier DR, Lloyd KC,Bradley A, Skarnes WC. Agouti C57BL/6N embryonic stem cells for mouse geneticresources. Nat Methods. 2009 Jul;6(7):493-5. doi: 10.1038/nmeth.1342. Epub 2009Jun 14. (Medline PMID: 19525957)

Colony and Husbandry Information Expand All

Special Considerations

These cells are expected to be male (XY), are feeder-dependent, and homozygous (a/a) for the a, non-agouti, coat color allele.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. The JM8 Parental Cell Line, from which these cells were derived, was tested for multiple mouse pathogens; please see the Health Status Report.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information Expand All

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the JM8 Parental MTA

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
050957-UCD-CTRL	KOMP High Passage Parental cell line JM8 Parental Cell Line (suitable for DNA extraction, control purposes; not for germline transmission)	$148.67 / $306.50 Non-Profit / For-Profit	Vial	Suitable for DNA extraction, control purposes but not for germline transmission.
050957-UCD-CELL	KOMP Low Passage Parental cell line JM8 Parental Cell Line (suitable for electroporation and germline transmission)	$740.70 / $1,441.18 Non-Profit / For-Profit	Vial	Suitable for a single electroporation and germline transmission.
050957-UCD-ULTRA	KOMP Ultra-low Passage Parental cell line JM8 Parental Cell Line (suitable for multiple electroporations and germline transmission)	$2,059.70 / $2,993.83 Non-Profit / For-Profit	Vial	Suitable for a multiple electroporation and germline transmission.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet