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Strain Detail Sheet

Published: 11/14/2025
Revised: 11/14/2025

Strain Name:
B6J.129-Rhotm1Jlem Cnga3tm1Biel/LrdrMmjax
Stock Number:
075953-JAX
Citation ID:
RRID:MMRRC_075953-JAX
Other Names:
Cnga3-/-; Rho-/-

Strain Information

Strain Quality Control

QUALITY CONTROL OF B6J.129-Rhotm1Jlem Cnga3tm1Biel/LrdrMmjax  |  The Jackson Laboratory
Last Updated:
Strain Data and Information Information by Submitter Assessed by MMRRC1,2
Published Provided
Allele-specific genotype3n.d.
Genetic backgroundn.d.
Viability of genotypes available for distribution n.d.
Specific Pathogen- Status n.d.
Recoverability of cryopreserved sperm/embryos4 n.d.
Gene or allele sequence3 n.d.
Gene or allele expression3n.d.
Gene or allele function3n.d.
Observable and/or measurable phenotypesn.d.
Fertility of genotypes available for distribution n.d.
Fecundity/breeding performance n.d.

1 When indicated as verified ("YES"), then please note that information presented is to the best of our knowledge correct and up-to-date at the time of verification at the MMRRC Distribution Center; however, this information is subject to change due to breeding, maintenance, and other actions on the mouse strain at the MMRRC Distribution Center; direct any questions on this table to the MMRRC Distribution Center for this mouse stain.

2 If verification has not been performed (as indicated by "NO"), investigators may request specific verification testing for a fee. Requests should be submitted directly to the MMRRC Distribution Center assigned to the management, archiving, and distribution of the strain. A full listing of available testing and analytical services is available at https://www.mmrrc.org/about/services.php.

3 This information may or may not apply to each individual engineered allele (e.g., Cre, FlpO) present in the strain.

4 Recovery refers to thawing, in vitro fertilization (IVF), and/or embryo culture leading to live offspring.

Gene Details

Rhotm1Jlem
Name: rhodopsin; targeted mutation 1, Janis Lem
Synonyms: mrho-, Opsin -, rhodopsin-
Type: Allele
Species: Mus musculus
Chromosome: 6
Alteration at locus: Targeted Mutation
Cnga3tm1Biel
Name: cyclic nucleotide gated channel alpha 3; targeted mutation 1, Martin Biel
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Targeted Mutation
Rho
Name: rhodopsin
Synonyms: Red Opsin, L opsin, LWS opsin, Long Wavelength Sensitive opsin, Rod Opsin, opsin 2, RP4, Opn2, Ops, Noerg1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 212541
Homologene: 68068

Additional Resources
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Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
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Cnga3
Name: cyclic nucleotide gated channel alpha 3
Synonyms: CNG3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12790
HGNC: HGNC:2150
Homologene: 994

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Cnga3tm1Biel: The Cnga3 gene was disrupted by replacement of exon 7 with a neomycin resistance cassette. Absence of gene expression in homozygous mutant animals was confirmed by RT-PCR analysis of retina RNA.

Rhotm1Jlem: A PGK-neo cassette was inserted into the first coding exon of the endogenous gene, deleting 15 bp upstream of the translational start site and 111 codons. Transcript was undetected in homozygous mutant mice by Northern blot and RT-PCR analyses. Western blot analysis indicated an absence of protein in whole retinal homogenates.
ES Cell Line
J1 (Rho) and R1(Cnga3)

Strain Description

Phenotype
B6J.129-Rhotm1JlemCnga3tm1Biel/LrdrMmjax (Cnga3-/-; Rho-/-), coneless and rodless double knockout mice, show no pupil response to low and medium-intensity red light. Pupil responses to other stimuli revealed a melanopsin-mediated contribution; however, a 40–60% reduction in maximal constriction amplitude indicated that rods, cones, and melanopsin all contribute to normal pupil constriction in wild-type mice. Similar to single knockout models, Cnga3-/-; Rho-/- mice showed an increased sust/max ratio to blue stimuli, indicating delayed pupil dilation in the later postillumination phase due to loss of classic photoreceptor function. Furthermore, a prolonged constriction to high-intensity red light is observed, likely driven by melanopsin activation. At one month of age, no rod nor cone function is detected by electroretinogram nor pupillometry in the Cnga3-/-; Rho-/- mice.

This model provides a valuable opportunity to study a retina that lacks rod and cone function, but still exhibits inner retinal photosensitivity,
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
MeSH Terms
  • Animals
  • Light
  • Mice
  • Models, Animal
  • Photic Stimulation
  • Pupil/physiology
  • Reflex, Pupillary/physiology
  • Retinal Cone Photoreceptor Cells/physiology
  • Retinal Ganglion Cells/cytology
  • Retinal Rod Photoreceptor Cells/physiology
  • Rod Opsins/metabolism
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Models for Human Disease
  • Neurobiology
  • Research Tools

Strain Origin

Submitter
Corinne Kostic, Ph.D., Jules-Gonin Eye Hospital, University of Lausanne.
Primary Reference

Kostic C, Crippa SV, Martin C, Kardon RH, Biel M, Arsenijevic Y, Kawasaki A. Determination of Rod and Cone Influence to the Early and Late Dynamic of the Pupillary Light Response. Invest Ophthalmol Vis Sci. 2016 May 1;57(6):2501-8. doi: 10.1167/iovs.16-19150. (Medline PMID: 27152964)

Additional References

Lem J, Krasnoperova NV, Calvert PD, Kosaras B, Cameron DA, Nicolò M, Makino CL, Sidman RL. Morphological, physiological, and biochemical changes in rhodopsin knockout mice. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):736-41. doi: 10.1073/pnas.96.2.736. (Medline PMID: 9892703)

Biel M, Seeliger M, Pfeifer A, Kohler K, Gerstner A, Ludwig A, Jaissle G, Fauser S, Zrenner E, Hofmann F. Selective loss of cone function in mice lacking the cyclic nucleotide-gated channel CNG3. Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7553-7. doi: 10.1073/pnas.96.13.7553. (Medline PMID: 10377453)

Strain Development
Cnga3tm1Biel: Cng3 DNA was isolated from a genomic P1 library made from 129/Sv mouse tissue. Three exons were identified corresponding to exons 5–7 of the CNG3 gene. A targeting vector was constructed and the herpes simplex thymidine kinase cassette was cloned 39 to the CNG3 sequence. The targeting vector was linearized and electroporated into R1 ES cells. G418/gancyclovir-resistant clones were screened by Southern blot analysis, and ES cells carrying the disrupted allele were microinjected into C57BL/6 blastocysts to produce chimeric mice. Chimeric mice were crossed with strains C57BL/6 (outbred) and 129/Sv (inbred). Heterozygotes then were intercrossed to produce mice homozygous for the CNG3 mutation.

Rhotm1Jlem: A targeting construct was made from a 129/Sv genomic clone. J1 embryonic stem cells were electroporated with the targeting construct and selected in G418 and gancyclovir. Of the 79 clones, 13 were identified as homologous recombinants via EcoRI digestion. In addition, six putative recombinants were verified by BamHI digestion.

Cnga3tm1Biel and Rhotm1Jlem were crossed and then backcrossed with C57BL/6J for at least 5 generations.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

For more information about this colony's health status contact csmmrrc@jax.org

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
>5
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
4 to 6
Recommended wean age
4 Weeks
Average Pups Weaned
4 to 6

Order Information

Availability Level

The availability level for this product has not been determined.

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contactThomas Wolfensberger.

Conditions of Distribution

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact Thomas Wolfensberger

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.