Strain Name:
B6.129P2-B3galt2tm1Dgen/Mmnc
Stock Number:
011602-UNC
Citation ID:
RRID:MMRRC_011602-UNC
Major Collection:

Strain Information

B3galt2tm1Dgen
Name: UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2; targeted mutation 1, Deltagen
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
B3galt2
Name: UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 26878
HGNC: HGNC:917
Homologene: 74512
Genetic Alterations
The endogenous gene promoter drives expression of inserted lacZ gene.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    E14 derived from 129P2/OlaHsd
    Phenotype
    Homozygous mutants displayed an increase in total distance traveled on the Open Field test.
    Homozygous mutants spent significantly more time in the central region in the Open Field test.
    Homozygous mutants spent significantly more time immobile in the Tail Suspension test.
    Homozygous mutants were impaired on the Rotarod test.
    Homozygous mutants displayed significantly increased acoustic startle responses.
    Phenotypic data provided by Deltagen, Inc. Full report available through MGI data link (includes genotyping information under "Molecular Biology" at top of screen).
    Mammalian Phenotype Terms
    Allelic Composition: B3galt2tm1Dgen/B3galt2tm1Dgen (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

    Strain Development
    ES cells derived from the 129/OlaHsd mouse substrain were used to generate chimeric mice. F1 mice were generated by breeding with C57BL/6 females. The resultant F1N0 heterozygotes were backcrossed to C57BL/6 mice to generate F1N1 heterozygotes. F2N1 homozygous mutant mice were produced by intercrossing F1N1 heterozygous males and females. Subsequent crosses made to C57BL/6.
    MMRRC Genetic QC Summary
    Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

    GQC analysis was done on one XY sample YK0365 at the time of importation and the results of the analysis are congruent with the SDS. The primary (host) background is C57BL/6. There is positive evidence of C57BL/6J and C57BL/6N substrains. The secondary (donor) background is consistent with 129P2, which is indicated in the submission and the SDS as the donor strain. The mitochondrial genome is consistent with both the host and donor background (identical by descent). The background of the Y chromosome (129P2) is not consistent with at least one backcross to the host genome. The region containing the allele of interest has the expected donor background (129P2). The sample is heterozygous for the donor background at the locus. The IRES construct is detected in the sample.

    The genome of the MMRRC: 11602 strain is 98.5 % inbred and 9.8% congenic at the strain level. 1.5% of the genome is segregating for the secondary (donor) background. 96.2% of the genome is segregating for the two substrains (C57BL/6J and C57BL/6N) that contribute to the primary (host) background. The contribution of C57BL/6J is 95.1%. The contribution of C57BL/6N is 4.9%.

    The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible.
    MiniMUGA Sample Report

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires execution of the Deltagen Standard Academic license agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    011602-UNC-EMBRYO Cryo-preserved embryos $1,404.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    011602-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    011602-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.