Strain Name:
B6;129P2-Gria4tm1Dgen/Mmnc
Stock Number:
011625-UNC
Citation ID:
RRID:MMRRC_011625-UNC
Major Collection:

Gene Information

Gria4tm1Dgen
Name: glutamate receptor, ionotropic, AMPA4 (alpha 4); targeted mutation 1, Deltagen
Synonyms: Gria4KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Gria4
Name: glutamate receptor, ionotropic, AMPA4 (alpha 4)
Synonyms: Glur-4, Gluralpha4, Glur4, spkw1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
NCBI: 14802
HGNC: HGNC:4574
Homologene: 20227
Genetic Alterations
The endogenous gene promoter drives expression of inserted lacZ gene.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    E14 derived from 129P2/OlaHsd
    Phenotype
    Behavior: Homozygous mutant mice exhibited increased total distance traveled during open field testing, an increase in thermal response latency during hot plate testing, and required a higher dose of metrazol to reach some seizure stages and a significantly lower dose to reach others.
    There were no other significant differences detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice.
    Phenotypic data provided by Deltagen, Inc. Full report available through MGI data link (includes genotyping information under "Molecular Biology" at top of screen).
    Mammalian Phenotype Terms
    Allelic Composition: Gria4tm1Dgen/Gria4tm1Dgen (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

    • behavior/neurological
    • nervous system
      • abnormal seizure response to pharmacological agent [MP:0000950]
    • integument
      • increased thermal nociceptive threshold [MP:0001973]
    Allelic Composition: Gria4tm1Dgen/Gria4tm1Dgen (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

    Allelic Composition: Gria4tm1Dgen/Gria4+ (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

    Allelic Composition: Gria4spkw1/Gria4tm1Dgen (Genetic Background: involves: 129P2/OlaHsd * C3H/HeJ * C57BL/6J )

    Strain Development

    ES cells derived from the 129/OlaHsd mouse substrain were used to generate chimeric mice. F1 mice were generated by breeding with C57BL/6 females. F2 homozygous mutant mice were produced by intercrossing F1 heterozygous males and females. Subsequent crosses made to C57BL/6.

    A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

    MMRRC Strain Reference Strains Genomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    B6;129P2-Gria4tm1Dgen 11625 C57BL/6J 129P2/OlaHsd 2446 0 136 0

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires execution of the Deltagen Standard Academic license agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    011625-UNC-EMBRYO Cryo-preserved embryos $1,363.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    011625-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.