Strain Name:
B6;129S5-Pik3cdGt(OST451938)Lex/Mmucd
Stock Number:
011755-UCD
Citation ID:
RRID:MMRRC_011755-UCD
Major Collection:

Gene Information

Pik3cdGt(OST451938)Lex
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta; gene trap OST451938, Lexicon Genetics
Synonyms: Pik3cdGt(neo)368Lex
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Gene Trap
Pik3cd
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Synonyms: 2610208K16Rik, p110delta, 2410099E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Gene Trap
NCBI: 18707
HGNC: HGNC:8977
Homologene: 3686
Genetic Alterations
The disrupted mouse gene is Pik3cd (phosphatidylinositol 3-kinase catalytic delta polypeptide), the ortholog of human PIK3CD. Aliases include p110delta, p110D, 2410099E07Rik, PI3-kinase p110 subunit delta, and PtdIns-3-kinase p110. Retroviral insertion disrupted the gene between coding exons 3 and 4.
Genotype Determination
ES Cell Line
Lexicon cell line derived from 129S5/SvEvBrd
Phenotype
The homozygous mutant mice exhibited increased median total white blood cell and absolute lymphocyte counts when compared with those of their wild-type littermates and the historical means. In addition, the homozygous mutants exhibited a decreased mean serum IgG1 response to ovalbumin challenge. RT-PCR analysis revealed that the transcript was absent in the homozygous mutant mice.
Phenotypic data provided by Lexicon Genetics, Inc. Full report available through MGI data link (includes genotyping information under "Genetics" on left side of screen).
Mammalian Phenotype Terms
Allelic Composition: Pik3cdGt(OST451938)Lex/Pik3cdGt(OST451938)Lex (Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J )

Strain Development
Targeted or gene trap mutations are generated in strain 129/SvEvBrd-derived embryonic stem (ES) cells. The chimeric mice are bred to C57BL/6J albino mice to generate F1 heterozygous animals. These progeny are intercrossed to generate F2 wild type, heterozygous, and homozygous mutant progeny. On rare occasions, for example when very few F1 mice are obtained from the chimera, F1 heterozygous mice are crossed to 129/SvEvBrd x C57BL/6 hybrid mice to yield additional heterozygous animals for the intercross to generate the F2 mice. Level I phenotypic analysis is performed on mice from this generation.
Suggested Control Mice
  • Wildtype littermates
Donor
Lexicon Genetics Incorporated

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires execution of the Lexicon Simple Letter Agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
011755-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
011755-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
011755-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.