Strain Name:
STOCK MipGt(OST1231)Lex/Mmmh
Stock Number:
015383-MU
Citation ID:
RRID:MMRRC_015383-MU
Other Names:
Major intrinsic protein

Strain Information

MipGt(OST1231)Lex
Name: major intrinsic protein of lens fiber; gene trap OST1231, Lexicon Genetics
Synonyms: AQP0-, Miptm1Lex, MipGt(Betageo)513Lex, MipGt(VICTR20)8Lex
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Gene Trap
Mip
Name: major intrinsic protein of lens fiber
Synonyms: Svl, MIP26, Cts, Aqp0, shrivelled, aquaporin 0, Lop, lens opacity
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Gene Trap
NCBI: 17339
HGNC: HGNC:7103
Homologene: 40627
Genetic Alterations
Gene trap vector inserted into exon 1.
Genotype Determination
ES Cell Line
Lexicon cell line derived from 129S5/SvEvBrd
Phenotype
Homozygous: Cataracts at eyes- open and microphthalmia

Heterozygous: Cataracts develop at ~6 months
Mammalian Phenotype Terms
Allelic Composition: MipGt(OST1231)Lex/MipGt(OST1231)Lex (Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J )

Allelic Composition: MipGt(OST1231)Lex/Mip+ (Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J )

MeSH Terms
  • Age Factors
  • Animals
  • Aquaporins
  • Cataract/genetics
  • Cataract/pathology
  • Cataract/physiopathology
  • Chimera
  • Crosses, Genetic
  • Disease Progression
  • Eye Proteins/genetics
  • Eye Proteins/metabolism
  • Gene Targeting
  • Genetic Predisposition to Disease
  • Heterozygote
  • Homozygote
  • Lasers
  • Lens, Crystalline/pathology
  • Lens, Crystalline/physiopathology
  • Membrane Glycoproteins/deficiency
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/metabolism
  • Mice
  • Mice, Knockout
  • Optics and Photonics
  • Osmosis
  • Permeability
  • Water/metabolism
Strain Development
Chimeric mice were derived from injections of OST1231 ES cells into C57BL/6J-Albino host blastocysts, and heterozygous mice from breeding high-contribution male chimeras to wild type C57BL/6J-Albino females. All intercrosses among heterozygous and homozygous animals were in a hybrid 129S5/C57BL/6J-Albino background. Mice were barrier-housed in 12hr light and dark cycles with free access to food and water in accordance with the animal studies regulations at Washington University.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Models for Human Disease
Donor
Alan Shiels, Ph.D., Washington University
Primary Reference
Shiels A, Bassnett S, Varadaraj K, Mathias R, Al-Ghoul K, Kuszak J, Donoviel D, Lilleberg S, Friedrich G, Zambrowicz B. Optical dysfunction of the crystalline lens in aquaporin-0-deficient mice. Physiol Genomics. 2001 Dec 21;7(2):179-86. (Medline PMID: 11773604)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires execution of the Lexicon Simple Letter Agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
015383-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
015383-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.