Strain Name:
B6.129S2-Ifnar1tm1Agt/Mmjax
Stock Number:
032045-JAX
Citation ID:
RRID:MMRRC_032045-JAX
Other Names:
A129, 010830, IFN-αβR-

Gene Information

Ifnar1tm1Agt
Name: interferon (alpha and beta) receptor 1; targeted mutation 1, Michel Aguet
Synonyms: IFNRI KO, IFN-IR KO, A 129, IFNRI -, IFN-IR-, IFN-alpha/betaR-, IFN-alpha/betaR0, IFN type I receptor, Ifnar1-, IFNR-, IFN-alphabeta-R KO, IFNAR-, IFNRA1,-., IFN Alpha Ro/o, A129, IFNAR(0), IFN-alpha/betaR/A129, IFN-alphabetaR-, A-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
Ifnar1
Name: interferon (alpha and beta) receptor 1
Synonyms: Ifrc, Ifar, IFN-alpha/betaR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
NCBI: 15975
HGNC: HGNC:5432
Homologene: 524
Genetic Alterations
A neomycin cassette was inserted into exon 3 at a position corresponding to amino acid 88.
Genotype Determination
ES Cell Line
D3
Phenotype
Homozygous: Mice that are homozygous for the targeted mutation are viable, fertile, and normal in size. No gene product (mRNA) is detected by Northern blot analysis of homozygous primary embryonic fibroblasts. Mice that are homozygous for this targeted allele lack type I IFN receptor function, exhibit enhanced osteoclastogenesis with decreased bone density, and impaired response to protozoan parasite (Leishmania) infection. NK cell mediated tumor regression does not occur in homozygotes in response to B16 melanoma tumor implant model. Mouse mutants involving this gene have been used in studies of Zika virus pathogenesis.

Heterozygous: Not characterized
Mammalian Phenotype Terms
Allelic Composition: Ifnar1tm1Agt/Ifnar1tm1Agt (Genetic Background: B6.129S2-Ifnar1tm1Agt )

Allelic Composition: Ifnar1tm1Agt/Ifnar1tm1Agt (Genetic Background: involves: 129S2/SvPas )

Allelic Composition: Ifnar1tm1Agt/Ifnar1tm1Agt (Genetic Background: involves: 129S2/SvPas * 129S6/SvEvTac )

Allelic Composition: Ifnar1tm1Agt/Ifnar1tm1Agt (Genetic Background: involves: 129S2/SvPas * BALB/c * C57BL/6 * SJL )

Allelic Composition: Ifnar1tm1Agt/Ifnar1tm1Agt (Genetic Background: involves: 129S2/SvPas * C57BL/6 )

MeSH Terms
  • Alphavirus Infections/immunology
  • Animals
  • Antibodies, Viral/biosynthesis
  • Disease Susceptibility
  • Immunity, Innate
  • Interferon Type I/physiology
  • Interferon-gamma/physiology
  • Lymphocytic Choriomeningitis/immunology
  • Membrane Proteins
  • Mice
  • Mutation
  • Receptor, Interferon alpha-beta
  • Receptors, Interferon/genetics
  • Receptors, Interferon/physiology
  • Rhabdoviridae Infections/immunology
  • Semliki forest virus
  • Signal Transduction
  • T-Lymphocytes/immunology
  • Vesicular stomatitis Indiana virus
  • Virus Diseases/immunology
Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 3. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The mice were backcrossed to C57BL/6 for at least 5 generations, as reported by the donating investigator.
Suggested Control Mice
Wild-type littermates
  • Human Diseases
    • Zika Virus
    Donor
    Michel Aguet, Ph.D., Swiss Institute for Experimental Cancer Research
    Primary Reference
    Muller U; Steinhoff U; Reis LF; Hemmi S; Pavlovic J; Zinkernagel RM; Aguet M, Functional role of type I and type II interferons in antiviral defense., Science 1994 Jun 24;264(5167):1918-21 (Medline PMID: 8009221)

    Colony and Husbandry Information

    When maintaining a live colony, these mice can be bred as homozygotes. SPF conditions are recommended.

    Colony's Current Health Status Report

    For more information about this colony's health status contact csmmrrc@jax.org

    Order Request Information

    Colony is sized to accommodate orders of approximately 10 mice within the age range of 4-8 weeks. Larger quantities may be available, please contact the distributing center directly for more details at csmmrrc@jax.org.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.



    This strain is only available for distribution within North America.Notice regarding distribution of this strain

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    032045-JAX-HOM-F
    032045-JAX-HOM-M
    Homozygous Female
    Homozygous Male
    $140.00 / $140.00
    Non-Profit / For-Profit
    Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
    032045-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.