Strain Name    : B6.129S2-Ifnar1tm1Agt/Mmjax

Stock Number : 032045-JAX

Gene Information

(provided by MGI)
Allele Symbol: Ifnar1tm1Agt
Name: targeted mutation 1, Michel Aguet
Chromosome: 16
Alteration at locus: Knockout
Gene Symbol: Ifnar1
Name: interferon (alpha and beta) receptor 1
Chromosome: 16
Alteration at locus: Knockout
Genetic Alterations:

Genotype Determination:

ES Cell Line:

Phenotype

Homozygous phenotype: Mice that are homozygous for the targeted mutation are viable, fertile, and normal in size. No gene product (mRNA) is detected by Northern blot analysis of homozygous primary embryonic fibroblasts. Mice that are homozygous for this targeted allele lack type I IFN receptor function, exhibit enhanced osteoclastogenesis with decreased bone density, and impaired response to protozoan parasite (Leishmania) infection. NK cell mediated tumor regression does not occur in homozygotes in response to B16 melanoma tumor implant model.

Heterozygous phenotype:

Mammalian Phenotype Terms:
Allelic Composition: Ifnar1tm1Agt/Ifnar1tm1Agt (Genetic Background: B6.129S2-Ifnar1tm1Agt )

Allelic Composition: Ifnar1tm1Agt/Ifnar1tm1Agt (Genetic Background: involves: 129S2/SvPas )

Allelic Composition: Ifnar1tm1Agt/Ifnar1tm1Agt (Genetic Background: involves: 129S2/SvPas * 129S6/SvEvTac )

Allelic Composition: Ifnar1tm1Agt/Ifnar1tm1Agt (Genetic Background: involves: 129S2/SvPas * BALB/c * C57BL/6 * SJL )

Allelic Composition: Ifnar1tm1Agt/Ifnar1tm1Agt (Genetic Background: involves: 129S2/SvPas * C57BL/6 )

Strain Development: A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 3. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The mice were backcrossed to C57BL/6 for at least 5 generations, as reported by the donating investigator.

Suggested Control Mice: Wild-type littermates

Donor: Michel Aguet, Ph.D., Swiss Inst. for Experimental Cancer Res.

Primary Reference:

Muller U; Steinhoff U; Reis LF; Hemmi S; Pavlovic J; Zinkernagel RM; Aguet M, Functional role of type I and type II interferons in antiviral defense., Science 1994 Jun 24;264(5167):1918-21 (Medline PMID: 8009221)

Colony and Husbandry Information

When maintaining a live colony, these mice can be bred as homozygotes. SPF conditions are recommended.



Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email csmmrrc@jax.org.

Order Request Information

The availability level for this product has not been determined.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
- Products for this strain are Not Yet Available for Ordering -
If you register interest in this strain, you will be notified when it becomes available for ordering.
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
032045-JAX-HOM-FHomozygous female
032045-JAX-HOM-MHomozygous male
$218.00 / $571.00
Non-Profit / For-Profit
Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

This strain is not currently available. If you register interest in this strain, you will be notified when it becomes available for ordering (click Register Interest button above).