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Strain Name:
B6.FVB(Cg)-Tg(Drd2-cre)ER44Gsat/Mmucd
Stock Number:
032108-UCD
Citation ID:
RRID:MMRRC_032108-UCD
Major Collection:

Strain Information

cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Drd2
Name: dopamine receptor D2
Synonyms: D2 receptor, D2R, Drd-2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13489
VEGA: 9
HGNC: HGNC:3023
Homologene: 22561
Tg(Drd2-cre)ER44Gsat
Name: transgene insertion ER44, GENSAT Project at Rockefeller University
Synonyms: Tg(Drd2-cre)44Gsat, ER44, Drd2-Cre, D2R Cre
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (cre)
Genetic Alterations

BAC engineering was used to insert an intron containing cre cassettes, followed by a polyadenylation sequence to terminate transcription of the fusion transcript immediately after the recombinase gene, into the BAC vector at the initiating ATG codon in the first coding exon of the gene. The BAC address for this line is RP23-161H15. SNP analysis has shown the transgenic insertion to be on chromosome 6 between SNPs rs13478819 and rs13459097.

Phenotype
This congenic strain carries the same mutation as MMRRC:017263 and was originally characterized on a STOCK background before backcrossing to C57BL/6J for at least 5 generations. It should be noted that the phenotype could vary from that originally described. BAC-transgenic images of the central nervous system at various ages of the transgenic mouse can be found on the GENSAT project page.
MeSH Terms
  • Animals
  • Axons/metabolism
  • Cell Differentiation
  • Cell Lineage
  • Cell Movement
  • Central Nervous System/cytology
  • Central Nervous System/metabolism
  • Chromosomes, Artificial, Bacterial/genetics
  • Cyclic Nucleotide Phosphodiesterases, Type 1
  • Gene Expression Profiling/methods
  • Gene Expression Regulation, Developmental
  • Gene Library
  • Genes, Reporter/genetics
  • Genetic Vectors/genetics
  • Green Fluorescent Proteins
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • LIM-Homeodomain Proteins
  • Luminescent Proteins/analysis
  • Luminescent Proteins/genetics
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/metabolism
  • Mice
  • Mice, Transgenic
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Neurosciences/methods
  • Phosphoric Diester Hydrolases/genetics
  • Phosphoric Diester Hydrolases/metabolism
  • Synapses/metabolism
  • Transcription Factors
  • Transgenes/genetics
Strain Development
The original transgenic mice were created in the GENSAT project in collaboration with the Intramural Program of the National Institute of Mental Health. The transgenic mice have a modified BAC-cre injected into pronuclei of FVB/N fertilized oocytes. Offspring of founders were crossed to a Soriano Rosa26/stop/EGFP reporter line. The MMRRC backcrossed this transgene from the mixed background to C57BL/6J selected for mice with only the cre expression, and eliminated the Rosa26-GFP from the line. Mice were determined to be congenic on C57BL/6J by SNP analysis.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Neurobiology
  • Research Tools
Donor
MMRRC at University of California, Davis. Made from the original strain (MMRRC:017263) donated by Nathaniel Heintz, Ph.D., The Rockefeller University, GENSAT and Charles Gerfen, Ph.D., National Institutes of Health, National Institute of Mental Health
Primary Reference

Gong S, Doughty M, Harbaugh CR, Cummins A, Hatten ME, Heintz N, Gerfen CR. Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs. J Neurosci. 2007 Sep 12;27(37):9817-23. (Full Text Journal Article).

Gong S, Zheng C, Doughty ML, Losos K, Didkovsky N, Schambra UB, Nowak NJ, Joyner A, Leblanc G, Hatten ME, Heintz N. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature. 2003 Oct 30;425(6961):917-25. (Medline PMID: 14586460).

Colony and Husbandry Information

In general, GENSAT BAC transgenic mouse lines should be utilized in the hemizygous state. Breeding of the BAC transgene to a homozygous state carries an increased risk of generating an unwanted phenotype for two reasons. First, the transgene inserts at random into the genome. Breeding the transgene to homozygosity results in mice that carry this insertion on both chromosomes, increasing the probability that the insertion will result in an observable phenotype. Second, BAC transgenes may carry passenger genes that are also expressed from the BAC vector. Breeding to homozygosity doubles the number of these genes carried in the transgenic mouse, increasing the possibility that a phenotype will result as a consequence of increased gene dosage. In our experience, observable phenotypes are rare in BAC transgenic mice when carried in the hemizygous state.

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Wild-type (C57BL/6J) female x Hemizygous Tg male or reciprocol mating
  • Wild-type female x Hemizygous Tg male sibling or reciprocol mating
Generation
N9. Strain was determined to be congenic on C57BL/6J by SNP analysis.
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
6
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@ucdavis.edu for more details.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032108-UCD-HET-F
032108-UCD-HET-M
032108-UCD-WT-F
032108-UCD-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
$272.50 / Non-Profit Per Mouse The mmrrc@ucdavis.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
032108-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
032108-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.