Strain Name:
B6;129-Tg(CAG-dre)1Afst/Mmucd
Stock Number:
032246-UCD
Citation ID:
RRID:MMRRC_032246-UCD
Other Names:
CAGG's-Dre, B6;129-Tg(CAG-dre)1Afst/Mmcd

Gene Information

Tg(CAG-dre)1Afst
Name: transgene insertion 1, A Francis Stewart
Synonyms: CAG-Dre, CAGGs-Dre
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
R1 ES cells were electroporated with linearized construct CAGGs-Dre-IRES-puro
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype

Homozygous: Mice express Dre recombinase under the CAGGs promotor

Hemizygous: Mice express Dre recombinase under the CAGGs promotor

MeSH Terms
  • Animals
  • Base Sequence
  • Cell Line
  • DNA Nucleotidyltransferases/metabolism
  • Embryonic Stem Cells/drug effects
  • Embryonic Stem Cells/metabolism
  • Escherichia coli/enzymology
  • Escherichia coli Proteins/metabolism
  • Gene Targeting
  • Genes, Reporter
  • Integrases/metabolism
  • Ligands
  • Mice
  • Mifepristone/pharmacology
  • Molecular Sequence Data
  • Plasmids/genetics
  • Progesterone/metabolism
  • Prokaryotic Cells/drug effects
  • Prokaryotic Cells/metabolism
  • Proteins/metabolism
  • RNA, Untranslated
  • Recombinant Fusion Proteins/metabolism
  • Recombinases/metabolism
  • Recombination, Genetic/drug effects
  • Recombination, Genetic/genetics
  • Reproducibility of Results
Strain Development
R1 ES cells were electroporated with linearized CAGGs-Dre-IRES-puro, mutant cells were injected into 8-cell embryos, chimeras were mated to C57BL/6 mice and pups screened by Southern Blot for germline transmission. Germline hets were mated to C57BL/6. Subsequent mice were sibling or other intercross mated.
Suggested Control Mice
Wild-type littermates
  • Models for Human Disease

Donor
Konstantinos Anastassiadis, Ph.D., Technische Universitaet Dresden.
Primary Reference
Anastassiadis K; Fu J; Patsch C; Hu S; Weidlich S; Duerschke K; Buchholz F; Edenhofer F; Stewart AF, Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice., Dis Model Mech 2009 Sep-Oct;2(9-10):508-15 (Medline PMID: 19692579)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black or agouti
MMRRC Breeding System
Backcross or Intra-strain Random Mating
Breeding Scheme(s)
  • Wild-type (C57BL/6) female x Hemizygous male or reciprocal mating
  • Wild-type female x Hemizygous male from the colony or reciprocal mating
Generation
N2
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032246-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
032246-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
032246-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.