Strain Name:
B6;129S5-Lrrc4ctm1Lex/Mmucd
Stock Number:
032444-UCD
Citation ID:
RRID:MMRRC_032444-UCD
Other Names:
Lrrc4c, UNQ292
Major Collection:

Gene Information

Allele Symbol: Lrrc4ctm1Lex (Mus musculus (mouse))
Name: leucine rich repeat containing 4C; targeted mutation 1, Lexicon Pharmaceuticals
Chromosome: 2
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:5007224
Gene Symbol: Lrrc4c (Mus musculus (mouse))
Name: leucine rich repeat containing 4C
Synonyms: 6430556C10Rik, netrin g1 ligand
Chromosome: 2
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:2442636
Genetic Alterations
The Lrrc4c gene consists of 3 exons. Coding exon 1 was targeted (NCBI accession AK048322.1) by homologous recombination.
Genotype Determination
ES Cell Line
Lexicon cell line derived from 129S5/SvEvBrd
Phenotype
Phenotypic analysis of the F2 mice indicates this mutation affects immunology and neurology.

Phenotypic data provided by Lexicon Genetics, Inc.
Mammalian Phenotype Terms
Allelic Composition: Lrrc4ctm1Lex/Lrrc4ctm1Lex (Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J )

  • homeostasis/metabolism
    • increased circulating interleukin-6 level [MP:0008596]
  • immune system
    • increased circulating interleukin-6 level [MP:0008596]
MeSH Terms
  • Animals
  • Membrane Proteins/genetics
  • Mice
  • Mice, Knockout
Strain Development
Targeted or gene trap mutations are generated in strain 129/SvEvBrd-derived embryonic stem (ES) cells. The chimeric mice are bred to C57BL/6-Tyrc-Brd albino mice albino mice to generate F1 heterozygous animals. These progeny are intercrossed to generate F2 wild type, heterozygous, and homozygous mutant progeny. On rare occasions, for example when very few F1 mice are obtained from the chimera, F1 heterozygous mice are crossed to 129/SvEvBrd x C57BL/6 hybrid mice to yield additional heterozygous animals for the intercross to generate the F2 mice.

Mice are archived at the sib-mated generation F2. Recovery will be performed using C57BL/6J donors unless otherwise requested.
Suggested Control Mice
Wild-type littermates
  • Developmental biology
  • Neurobiology
Donor
Genentech, Inc.
Primary Reference
Tang T, Li L, Tang J, Li Y, Lin W, Martin F, Deanna Grant D, Solloway M, Parker L, Weilan Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ. A mouse knockout library for secreted and transmembrane proteins. Nat Biotechnol. 2010 July; 28(7):749–755. (Medline PMID: 20562862)

Colony and Husbandry Informationon

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Agouti or Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male
  • Heterozygous female x Heterozygous male
  • Homozygous female x Homozygous male
Generation
F2
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Undetermined
Average litter size
Unspecified
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires execution of the Genentech Lexicon agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032444-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
032444-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2
032444-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.