Strain Detail Sheet

Strain Name:
B6(Cg)-Cux2tm2.1(cre)Mull/Mmmh
Stock Number:
032778-MU
Citation ID:
RRID:MMRRC_032778-MU
Major Collection:
Neuroscience Blueprint

Strain Information

Gene Details

Cux2tm2.1(cre)Mull
Name: cut-like homeobox 2; targeted mutation 2.1, Ulrich Mueller
Synonyms: Cux2-Cre
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Targeted Mutation
cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Alteration at locus: Targeted Mutation
Cux2
Name: cut-like homeobox 2
Synonyms: Cutl2, Cux-2, 1700051K22Rik, ENSMUSG00000072641, Cux2
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Targeted Mutation
NCBI: 13048
Homologene: 22426
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genotype Determination
ES Cell Line
Not specified

Strain Description

Phenotype
Homozygous phenotype: The Cux2-Cre "knock-in" allele has a nuclear-localized Cre recombinase (NLS-Cre) inserted into the first coding ATG of the cut-like homeobox 2 (Cux2) locus; this was designed to both abolish Cux2 gene function and place NLS-Cre expression under the control of the Cux2 promoter/enhancer elements. Heterozygous mice are viable and fertile with no reported gross physical abnormalities. Although other mice with homozygous deletion of Cux2 gene function are prenatal lethal with neural tube development abnormalities, the donating investigator reports that these Cux2-Cre homozygous mice are viable with no reported gross physical abnormalities (homozygous fertility has not yet been characterized). The donating investigator has not determined if this Cux2-Cre allele abolishes expression of a functional Cux2 mRNA/protein, or if it retains expression of some functional splice variant of Cux2. The donating investigator reports Cre recombinase activity is observed throughout the central nervous system, including most brain regions and spinal cord. When tested in the entire embryo at embryonic day (E)12.5, the donating investigator reports that Cre recombinase activity seemed limited to the central nervous system. When bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Cux2-expressing cells in the offspring. These Cux2-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of tissues throughout the central nervous system, including most brain regions and spinal cord.
Strain Development
A targeting vector was designed to insert a nuclear-localized Cre recombinase gene and polyA signal followed by an FRT-flanked PGK-Neo cassette into the initiation codon of the cut-like homeobox 2 (Cux2) locus. This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6 mice to establish the colony. Next, mutant mice were bred with FLPe-expressing mice on a C57BL/6 congenic background to remove the FRT-flanked PGK-Neo cassette. The resulting Cux2-Cre mice were then bred to C57BL/6J inbred mice for approximately two generations (selecting away from the FLPe transgene) prior to arrival at The Jackson Laboratory.
Suggested Control Mice
  • Wildtype littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Neurobiology
  • Research Tools

Strain Origin

Donor
Ulrich Mueller, Ph.D., The Scripps Research Institute.
Primary Reference
Additional information regarding this strain can be found at NIH Neuroscience Blueprint Cre Driver Network.

Colony and Husbandry Information

Special Considerations

When maintained as a live colony, heterozygous mice may be bred together, to wildtype mice from the colony, or to C57BL/6J inbred mice. Although other mice with homozygous deletion of Cux2 gene function are prenatal lethal with neural tube development abnormalities, the donating investigator reports that these Cux2-Cre homozygous mice are viable with no reported gross physical abnormalities (homozygous fertility has not yet been characterized).

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@missouri.edu

Order Request Information

Availability Level

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@missouri.edu for more details.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
032778-MU-HET-F
032778-MU-HET-M
032778-MU-WT-F
032778-MU-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
 $290.00 / Non-Profit Per Mouse The mmrrc@missouri.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
032778-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.