Strain Name:
B6.129S-Il21tm1Lex/Mmucd
Stock Number:
032800-UCD
Citation ID:
RRID:MMRRC_032800-UCD
Other Names:
IL-21-/-, B6.129S-Il21tm1Lex/Mmcd
Major Collection:

Gene Information

Il21tm1Lex
Name: interleukin 21; targeted mutation 1, Lexicon Genetics
Synonyms: IL-21-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
Il21
Name: interleukin 21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
NCBI: 60505
HGNC: HGNC:6005
Homologene: 11032
Genetic Alterations
Mice are IL-21-/- from the MMRRC (MMRRC:011723) backcrossed to C57BL/6. SNP analysis has shown the mutation to be on chromosome 3 between SNP IDs rs13476969 and rs6274061.
Genotype Determination
ES Cell Line
Lexicon cell line derived from 129S5/SvEvBrd
Phenotype
Homozygous: IL-21 deficient mice develop normally but develop a variety of immune system defects.
Heterozygous: There may be a gene dosage effect.
Phenotypic data provided by Lexicon Genetics, Inc. Full report available through MGI data link (includes genotyping information under "Genetics" on left side of screen).
Mammalian Phenotype Terms
Allelic Composition: Il21tm1Lex/Il21tm1Lex (Genetic Background: involves: 129S5/SvEvBrd * C57BL/6 )

Allelic Composition: Il21tm1Lex/Il21tm1Lex (Genetic Background: involves: 129S5/SvEvBrd * C57BL/6J )

MeSH Terms
  • Animals
  • CD4-Positive T-Lymphocytes/immunology
  • CD8-Positive T-Lymphocytes/immunology
  • Chronic Disease
  • Interleukins/immunology
  • Lymphocytic Choriomeningitis/immunology
  • Lymphocytic choriomeningitis virus
  • Mice
  • Mice, Inbred C57BL
  • Viral Load
Strain Development
The mice were originally obtained from the MMRRC (MMRRC:011723). They were on a mixed B6;129 background and were backcrossed 10-12 generations on to in house bred C57BL/6J mice. Mice were confirmed congenic on C57BL/6J by SNP analysis.
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Immunology and Inflammation
  • Virology
Donor
Allan Zajac, Ph.D., University of Alabama at Birmingham
Primary Reference
Yi JS; Du M; Zajac AJ, A vital role for interleukin-21 in the control of a chronic viral infection., Science 2009 Jun 19;324(5934):1572-6 (Medline PMID: 19443735)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • C57BL/6J female x Heterozygous male or reciprocal mating
Generation
N10+
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires execution of the Lexicon Simple Letter Agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032800-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
032800-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.