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Strain Detail Sheet

Strain Name:
B6;129-Mycntm1Psk Myctm2Fwa cDKO2 mES cells
Stock Number:
036284-JAX
Citation ID:
RRID:MMRRC_036284-JAX

Gene Information

Gene Details

Myctm2Fwa
Name: myelocytomatosis oncogene; targeted mutation 2, Frederick W Alt
Synonyms: Myclox, MYCfl, c-mycfl, Mycflox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Conditional
Mycntm1Psk
Name: v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived; targeted mutation 1, Paul S Knoepfler
Synonyms: Mycnloxp, N-mycFL, Mycntm1Rne, NmycFl
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Conditional
Mycn
Name: v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
Synonyms: Nmyc, N-myc, Nmyc-1, Nmyc1, bHLHe37
Type: Gene
Species: Mouse
Chromosome: 12
Alteration at locus: Conditional
NCBI: 18109
HGNC: HGNC:7559
Homologene: 3922
Myc
Name: myelocytomatosis oncogene
Synonyms: c-myc, Nird, Niard, Myc2, bHLHe39
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: Conditional
NCBI: 17869
HGNC: HGNC:7553
Homologene: 31092
Genetic Alterations

A loxP site flanked targeting vector containing a neomycin resistance gene under the control of the phosphoglycerate kinase, PGK, promoter was utilized in the construction of Mycntm1Psk. This selection cassette was inserted downstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into 129 derived embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette.

The Myctm2Fwa targeting vector was designed to place a loxP site between exons 1 and 2 and a loxP-flanked PGKneo cassette downstream of exon 3. The construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. The neomycin cassette was removed by transient transfection with a Cre recombinase expressing plasmid leaving loxP sites flanking exons 2 and 3.

ES Cell Line
Not specified

Strain Origin

Donor
Laura Reinholdt, Ph.D., The Jackson Laboratory
Primary Reference

Varlakhanova N, Cotterman R, deVries W, Morgan J, Donahue LR, Murray S, Knowles B, Knoepfler P. myc maintains embryonic stem cell pluripotencaand self-renewal. Differentiation 2010; 80:9-19. (Medline PMID: 20537458).

Colony and Husbandry Information

Special Considerations

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email csmmrrc@jax.org.

Order Request Information

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
036284-JAX-CELL B6;129-Mycntm1Psk Myctm2Fwa cDKO2 mES cells $546.00 / $546.00
Non-Profit / For-Profit		 Vial Includes genetic confirmation
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.