Strain Detail Sheet

Strain Name:
C57BL/6N-H3f3btm1Psk AC368 mES cells
Stock Number:
036926-JAX
Citation ID:
RRID:MMRRC_036926-JAX
Other Names:
C57BL/6N-H3f3btm1Psk/Mmjax AC368 mES cells, AC368 mES cells

Gene Information

Gene Details

H3f3btm1Psk
Name: H3.3 histone B; targeted mutation 1, Paul Knoepfler
Synonyms: H3f3bFL
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Conditional
Genetic Alterations
A targeting vector containing an FRT-flanked neomycin resistance cassette 3' of exon 1 followed by loxP sites flanking exons 2-4.
Genotype Determination
ES Cell Line
JM8.F6 derived from C57BL/6N

Strain Description

Strain of Origin
C57BL/6N

Related Protocols

Strain Origin

Donor
Laura Reinholdt, The Jackson Laboratory.
Primary Reference
Bush KM, Yuen BT, Barrilleaux BL, Riggs JW, O Geen H, Cotterman R, Knoepfler PS. Endogenous mammalian histone H3.3 exhibits chromatin-related functions during development. Epigenetics Chromatin. 2013 Apr 9;6(1):7. [Epub ahead of print] (Medline PMID: 23570311).

Colony and Husbandry Information

Special Considerations

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email csmmrrc@jax.org.

Order Request Information

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
036926-JAX-CELL C57BL/6N-H3f3btm1Psk AC368 mES cells $546.00 / Non-Profit Vial Includes genetic confirmation
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.