Strain Name:
C57BL/6N-Atm1Brd Pkd2l2tm1a(EUCOMM)Wtsi/HMmucd
Stock Number:
037865-UCD
Citation ID:
RRID:MMRRC_037865-UCD
Major Collection:

Strain Information

Pkd2l2tm1a(EUCOMM)Wtsi
Name: polycystic kidney disease 2-like 2; targeted mutation 1a, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Knockout-first
Pkd2l2
Name: polycystic kidney disease 2-like 2
Synonyms: Polycystin - L2, TRPP5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Knockout-first
NCBI: 53871
VEGA: 18
HGNC: HGNC:9012
Homologene: 22812
Genetic Alterations
Cassette L1L2_Bact_P inserted at chromosome 18 position 34414025. LoxP site at position 34414961.

Allele Detail page

KOMP Targeting Strategies

Genotype Determination
ES Cell Line
JM8A3.N1 derived from C57BL/6N
Phenotype
Phenotyping data may be available at mousephenotype.org.
MeSH Terms
  • Alleles
  • Animals
  • Computational Biology
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Gene Deletion
  • Gene Knockout Techniques/methods
  • Genes/genetics
  • Genes, Lethal/genetics
  • Genetic Association Studies/methods
  • Genetic Vectors/genetics
  • Genome/genetics
  • Genomics
  • Genotype
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout/genetics
  • Mutagenesis, Insertional/methods
  • Phenotype
  • Polymerase Chain Reaction
  • Rats
Strain Development
ES cell clone EPD0354_1_A07 was injected into C57BL/6J-Tyrc-Brd blastocysts. Resulting chimeras were mated to C57BL/6NTac, progeny were subsequently mated to C57BL/6NTac
Suggested Control Mice
Wild-type littermate or C57BL/6N mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
Donor
Martin Fray, Ph.D., MRC Harwell.
Mice were generated at MRC Harwell as part of the Baylor College of Medicine, Sanger Institute, and MRC Harwell (BaSH) Consortium for the NIH Common Fund program for Knockout Mouse Production and Cryopreservation (1U42RR033192-01) and Knockout Mouse Phenotyping (1U54HG006348-01).
Primary Reference
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A. A conditional knockout resource for the genome-wide study of mouse gene function. Nature, 2011, 474 (7351) 337-42. (Medline PMID: 21677750)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Agouti or black
MMRRC Breeding System
Intra-strain Random Mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male from the colony or reciprocal mating
Overall Breeding Performance
Comments by Donor. Generally heterozygous mice from this colony conform to normal expectations of the background strain. For maintenance of our colonies we pay particular attention to the age of the mating pairs and the resulting litters. In our experience the C57BL/6N substrain used to establish and progress this colony has shown some characteristics such as poor breeding, high pre-weaning mortality rates and failure to breed beyond three litters. We believe disturbance of litters has a detrimental effect on the mating pair. For our core and mutant colonies we have actively reduced our intervention with the mice. Daily observations, health checks, cleaning and cage movement is minimised in litters under 14 days of age.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the BaSH EUCOMM Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037865-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
037865-UCD-RESUS Litter recovered from cryo-archive $5,055.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.