Strain Detail Sheet

Strain Name:
C57BL/6J-Dclre1cm1Btlr/Mmmh
Stock Number:
038152--TBD-
Citation ID:
RRID:MMRRC_038152--TBD-
Other Names:
kiwis
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Dclre1cm1Btlr
Name: DNA cross-link repair 1C; mutation 1, Bruce Beutler
Synonyms: kiwis
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
Dclre1c
Name: DNA cross-link repair 1C
Synonyms: 9930121L06Rik, Artemis, Art
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227525
Homologene: 32547
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Genetic Alterations
ENU-induced A to G transition at base pair 3,436,475 (v38) on chromosome 2, or base pair 12,684 in the GenBank genomic region NC_000068 for the Dclre1c gene. The mutation corresponds to residue 431 in the mRNA sequences NM_146114, NM_175683, NM_001110214 within exon 5 of 14, 16, and 15 total exons, respectively. The mutation results in a histidine (H) to arginine (R) substitution at position 115 (H115R) in the Artemis protein.

Strain Description

Phenotype

Homozygous: Mice showed a decreased frequency of B cells and a decreased frequency of T cells including both CD4+ and CD8+ T cells as well as an increased frequency of macrophages, neutrophils, and natural killer (NK) cells, all in the peripheral blood. The T-independent antibody response to 4-hydroxy-3-nitrophenylacetyl-Ficoll (NP-Ficoll) was also diminished.

Heterozygous: No signficant abnormal phenotype detected.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation
  • Models for Human Disease
    • Severe combined (T-B-NK+) immunodeficiency associated with increased radiosensitivity (RS-SCID; OMIM: #602450)
    • Athabascan SCID (SCID-A; OMIM:#602450)
    • Omenn syndrome (OS; OMIM: #603554)

Strain Origin

Primary Reference

Wang K-W, Choi JH, Zeng M, Murray AR, Beutler B. Record for kiwis. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

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