Strain Detail Sheet

Strain Name:
C57BL/6J-Tlr4Lps-3Btlr/Mmmh
Stock Number:
038156--TBD-
Citation ID:
RRID:MMRRC_038156--TBD-
Other Names:
Lops
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Tlr4Lps-3Btlr
Name: toll-like receptor 4; defective lipopolysaccharide response 3 Bruce Beutler
Synonyms: Lops
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
Tlr4
Name: toll-like receptor 4
Synonyms: lipopolysaccharide response, Lps, Rasl2-8
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 21898
Homologene: 41317
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Genetic Alterations
ENU-induced T to A transversion at base pair 66,833,880 (v38) on chromosome 4, or base pair 6,070 in the GenBank genomic region NC_000070 for the Tlr4 gene. The mutation lies in the splice acceptor site of intron 1 of the 3 exon gene, and affects a thymine base 14 nucleotides from the next exon. One possibility, shown below, is that aberrant splicing may result in skipping of the 167 bp exon 2, utilization of the acceptor splice site from intron 2, and splicing of exon 1 to exon 3. Deletion of exon 2 would result in a frame-shift and the coding of one aberrant amino acid followed by a premature stop codon (the second abnormal codon after exon 1).

Strain Description

Phenotype

Homozygous: Mice showed some of which exhibited decreased TNFα secretion in response to Toll-like receptor 4 (TLR4) ligand LPS and impaired peritoneal macrophage inflammatory responses (i.e., decreased secretion of the proinflammatory cytokine interleukin (IL)-1β) following exposure to lipopolysaccharide (LPS) and aluminum adjuvants.

Heterozygous: Mice exhibited decreased TNFα secretion in response to Toll-like receptor 4 (TLR4) ligand LPS and impaired peritoneal macrophage inflammatory responses (i.e., decreased secretion of the proinflammatory cytokine interleukin (IL)-1β) following exposure to lipopolysaccharide (LPS) and aluminum adjuvants. Both phenotypes intermediate to homozygous Lops and wild-type mice.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • immune system
    • decreased interferon-beta secretion [MP:0008565]
    • decreased tumor necrosis factor secretion [MP:0008561]
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Primary Reference

Zhang Z, Wang Y, Shi H, Murray AR, Beutler B. Record for Lops. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

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