Strain Detail Sheet

Strain Name:
C57BL/6J-Lystbg-5Btlr/Mmmh
Stock Number:
038166--TBD-
Citation ID:
RRID:MMRRC_038166--TBD-
Other Names:
Swallow
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Lystbg-5Btlr
Name: lysosomal trafficking regulator; biege 5 Bruce Beutler
Synonyms: Swallow
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
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Genetic Alterations
ENU-induced T to C transition at base pair 13,757,422 (v38) on chromosome 13, or base pair 167,091 in the GenBank genomic region NC_000079 for the Lyst gene. The mutation corresponds to residue 10,815 in the mRNA sequence NM_010748.2 within exon 47 of 53 total exons. The mutation results in a phenylalanine (F) to serine (S) substitution at position 3545 (F3545S) in the Lyst protein.

Strain Description

Phenotype

Homozygous: Mice showed an increased rate of phagocytosis in peritoneal exudate cells (PECs). Phagocytosis was detected by measuring the fluorescence intensity of pHrodo Green E. coli conjugates two hours after treatment of PECs with an experimental phagocytosis effector (e.g., Cytochalasin D). The pHrodo Green E. coli conjugates fluoresce at acidic pH, such as in phagosomes.

Heterozygous: showed an increased rate of phagocytosis in peritoneal exudate cells (PECs). The heterozygote phenotype was intermediate to the wild-type and homozygous Swallow phenotype.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • immune system
  • hematopoietic system
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Primary Reference

Zhang Z, Wang Y, Shi H, Dao D, Sun L, Murray AR, Beutler B. Record for Swallow. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable:
Homozygotes are fertile:
Heterozygotes are fertile:
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

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Fees

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