Strain Detail Sheet

Strain Name:
C57BL/6J-ElaneM1Btlr/Mmmh
Stock Number:
038170--TBD-
Citation ID:
RRID:MMRRC_038170--TBD-
Other Names:
Ruo
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

ElaneM1Btlr
Name: elastase, neutrophil expressed; mutation 1, Bruce Beutler
Synonyms: Ruo
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
Elane
Name: elastase, neutrophil expressed
Synonyms: Ela2, NE, F430011M15Rik
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 50701
VEGA: 10
HGNC: HGNC:3309
Homologene: 20455
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Genetic Alterations
ENU-induced A to G transition at base pair 79,887,108 (v38) on chromosome 10, or base pair 797 in the GenBank genomic region NC_000076 for the Elane gene. The mutation corresponds to residue 370 in the mRNA sequence NM_015779 within exon 4 of 5 total exons. The mutation results in an aspartic acid (D) to glycine (G) substitution at position 116 (D116G) in the neutrophil elastase protein.

Strain Description

Phenotype

Homozygous: Mice showed decreased TNFα secretion in response to the Toll-like receptor 4 (TLR4) ligand LPS.

Heterozygous: Mice showed decreased TNFα secretion in response to the Toll-like receptor 4 (TLR4) ligand LPS; the heterozygote phenotype is highly variable and intermediate to the homozygous Ruo and wild-type mice.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • immune system
    • decreased tumor necrosis factor secretion [MP:0008561]
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Primary Reference

Zhang Z, Wang Y, Shi H, Murray AR, Beutler B. Record for ruo. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

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