Strain Detail Sheet

Strain Name:
C57BL/6J-GckM1Btlr/Mmmh
Stock Number:
038191--TBD-
Citation ID:
RRID:MMRRC_038191--TBD-
Other Names:
Grahamcracker
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

GckM1Btlr
Name: glucokinase; mutation 1, Bruce Beutler
Synonyms: Grahamcracker
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
Gck
Name: glucokinase
Synonyms: hexokinase 4, MODY2, HK4, Gls006, Hlb62
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 103988
HGNC: HGNC:4195
Homologene: 55440
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Genetic Alterations
ENU-induced T to A transversion at base pair 5,902,165 (v38) on chromosome 11, or base pair 47,917 in the GenBank genomic region NC_000077 for the Gck gene. The mutation corresponds to residue 1,655 in the mRNA sequence NM_010292 (variant 1) within exon 9 of 10 total exons and to residue 1,297 in the mRNA sequence NM_001287386 (variant 2) within exon 9 of 10 total exons. he mutation results in an asparagine (N) to lysine (K) substitution at position 391 (N391K) in both isoforms of the glucokinase (GCK) protein.

Strain Description

Phenotype

Homozygous: Mice exhibited impaired glucose tolerance (i.e., hyperglycemia) 30 minutes after glucose administration.

Heterozygous: Mice exhibited impaired glucose tolerance (i.e., hyperglycemia) 30 minutes after glucose administration; phenotype is intermediate between wild-type and homozygous Grahamcracker mice.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • homeostasis/metabolism
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Metabolism
  • Endocrine Deficiency
  • Diabetes
  • Models for Human Disease
    • Gestational diabetes mellitus [OMIM: #125851]
    • Late onset noninsulin-dependent diabetes mellitus [OMIM: #125853]
    • Permanent neonatal diabetes mellitus [OMIM: #606176]
    • Familial hyperinsulinemic hypoglycemia 3 [OMIM: #602485]
    • Type II maturity-onset diabetes of the young [MODY 2 (alternatively, GCK-MODY); OMIM: #125851]

Strain Origin

Primary Reference

Chen Z, SoRelle J, Hutchins N, Murray AR, Beutler B. Record for Grahamcracker. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

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