ENU-induced G to T transition at base pair 95,958,671 (v38) on chromosome 5, or base pair 1,733 in the GenBank genomic region NC_000071 for the ice gene. The mutation corresponds to residue 133 in the mRNA sequence NM_018866 within exon 2 of 4 total exons. The mutation results in a cysteine (C) to phenylalanine (F) substitution at position 34 (C34F) in the CXC chemokine ligand (CXCL) 13 [alternatively, B cell-attracting chemokine-1 (BCA-1) or B lymphocyte chemoattractant (BLC)] protein.

Homozygous: Mice showed an increase in B1 cell frequency in the peripheral blood and a diminished T-dependent antibody response to recombinant Semliki Forest virus (rSFV)-β-galactosidase (rSFV-β-gal).

Heterozygous: No abnormal phenotype detected.

Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.

Wild-type littermates or C57BL/6J mice

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.