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Strain Detail Sheet

Strain Name:
C57BL/6J-Tlr4lps-4Btlr/Mmmh
Stock Number:
038214--TBD-
Citation ID:
RRID:MMRRC_038214--TBD-
Other Names:
bugsy
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Tlr4lps-4Btlr
Name: toll-like receptor 4; defective lipopolysaccharide response 4 Bruce Beutler
Synonyms: bugsy
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
Tlr4
Name: toll-like receptor 4
Synonyms: Lps, Rasl2-8, lipopolysaccharide response
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 21898
Homologene: 41317

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced A to T transversion at base pair 66,839,254 (v38) on chromosome 4, or base pair 11,444 in the GenBank genomic region NC_000070 for the Tlr4 gene. The mutation corresponds to residue 564 in the mRNA sequence NM_021297 within exon 3 of 3 total exons. The mutation results in substitution of lysine (K) 95 to a premature stop codon (K95*) in the TLR4 protein.

Strain Description

Phenotype

Homozygous: Mice showed reduced TNFα secretion from macrophages in response to the Toll-like receptor 4 (TLR4) ligand, lipolysaccharide (LPS) and resistance to LPS-induced macrophage necroptosis.

Heterozygous: Mice showed reduced TNFα secretion from macrophages in response to the Toll-like receptor 4 (TLR4) ligand, lipolysaccharide (LPS); phenotype is intermediate between wild-type and homozygous bugsy mice.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • hematopoietic system
    • decreased macrophage cytokine production [MP:0011079]
  • immune system
    • decreased macrophage cytokine production [MP:0011079]
    • decreased tumor necrosis factor secretion [MP:0008561]
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Primary Reference

Wang Y, Shi H, Zhang Z, Sun L, Dao D, Murray AR, Beutler B. Record for bugsy. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

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