Loading...
Strain Detail Sheet
Strain Name:
C57BL/6J-Nos3m1Btlr/Mmmh
Stock Number:
038230--TBD-
Citation ID:
RRID:MMRRC_038230--TBD-
Other Names:
paul
Major Collection:
Nos3m1Btlr
Name: nitric oxide synthase 3, endothelial cell; mutation 1, Bruce Beutler
Synonyms: paul
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
MGI: MGI:5691955
Nos3
Name: nitric oxide synthase 3, endothelial cell
Synonyms: eNOS, ecNOS, Nos-3, 2310065A03Rik
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Chemically Induced
MGI: MGI:97362
NCBI: 18127
Ensembl: ENSMUSG00000028978
VEGA: OTTMUSG00000024082
HGNC: HGNC:7876
Homologene: 504
CCDS: CCDS19117.1
UCSC: Chr5:24569772-24589472
Additional Resources
Genetic Alterations
ENU-induced C to T transition at base pair 24,372,704 (v38) on chromosome 5, or base pair 7,886 in the GenBank genomic region NC_000071 for the Nos3 gene, corresponding to residue 1,734 in the mRNA sequence NM_008713 within exon 13 of 26 total exons. The mutation results in a threonine (T) to isoleucine (I) substitution at position 572 (T572I) in NOS3 protein.
Phenotype
Homozygous: Mice were susceptible to low doses of DSS (1%) and showed weight loss seven days after DSS treatment.
Heterozygous: No abnormal phenotype detected.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )- digestive/alimentary system
- increased susceptibility to induced colitis [MP:0008537]
- growth/size/body
- increased susceptibility to weight loss [MP:0010180]
- immune system
- increased susceptibility to induced colitis [MP:0008537]
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.
- Cell Biology
- Immunology and Inflammation
- Apoptosis
- Models for Human Disease
- Inflammatory bowel disease
Primary Reference
Turer E, Murray AR, Beutler B. Record for paul. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Coat Color
Black
| Viability and Fertility: | Female | Male | Comments |
|---|---|---|---|
| Homozygotes are viable: | Yes | Yes | |
| Homozygotes are fertile: | Yes | Yes | |
| Heterozygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | Unknown | Unknown |
The availability level for this product has not been determined.
The donor or their institution limits the distribution to non-profit institutions only.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the
Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.