Strain Detail Sheet

Strain Name:
C57BL/6J-Itchm1Btlr/Mmmh
Stock Number:
038232--TBD-
Citation ID:
RRID:MMRRC_038232--TBD-
Other Names:
scratch
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Itchm1Btlr
Name: itchy, E3 ubiquitin protein ligase; mutation 1, Bruce Beutler
Synonyms: scratch
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 6720481N21Rik, C230047C07Rik, AIP4, 8030492O04Rik
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16396
Homologene: 88442
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Genetic Alterations
ENU-induced T to C transition at base pair 155,172,561 (v38) on chromosome 2, or base pair 39,183 in the GenBank genomic region NC_000068 for the Itch gene, corresponding to residue 520 in the mRNA sequence NM_008395 (variant 1) within exon 5 of 25 total exons and residue 722 in the mRNA sequence NM_001243712 (variant 2) within exon 6 of 26 total exons. The mutation results in a leucine (L) to serine (S) substitution at position 106 (L106S) in both variants of the Itch protein.

Strain Description

Phenotype

Homozygous: Mice exhibited increased total IgE levels in the peripheral blood and an enhanced IgE response to the cysteine protease allergen, papain.

Heterozygous: No abnormal phenotype detected.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Cell Biology
  • Immunology and Inflammation

Strain Origin

Primary Reference

SoRelle J, Yue T, Murray AR, Beutler B. Record for scratch. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable:
Homozygotes are fertile:
Heterozygotes are fertile:
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

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